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Langerhans Cells from Oral Epithelium are More Effective in Stimulating Allogeneic T-cells in vitro than Langerhans Cells from Skin Epithelium

Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

M. Jontell

Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

G. Bergenholtz

Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

C. Eklund

Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

U.I. Dahlgren

Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden, Department of Clinical Immunology, Faculty of Odontology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

Dendritic cells, such as Langerhans cells (LC), in different ectodermal compartments may have different functional capabilities. The present study was undertaken to compare oral Langerhans cells (LC) with those of the epidermis in terms of their ability to co-stimulate T-cells in vitro. A Mixed Epithelial Cell Lymphocyte Reaction (MELR) and a mitogen-driven (concanavalin A) T-cell proliferation assay were used. In both assays, LC in a crude cell suspension of freshly isolated oral epithelial cells were found to be five times more effective in mediating T-cell proliferation than freshly isolated epidermal LC. Twenty-four-hour cell culture at 37°C enhanced the T-cell response in the MELR compared with cells cultured at 4°C. This applied to both skin and oral epithelial cells. Oral and skin epithelial cell suspensions depleted of LC lost the capacity to stimulate allogeneic T-cells. Incubation of the epithelial cell suspensions with recombinant Granulocyte/Macrophage-Colony Stimulating Factor (rGM-CSF) did not enhance the co-stimulating capacity of the LC. Titration of different numbers of oral and skin LC to T-cells showed that skin LC were never able to reach more than 44% of the maximal stimulatory capacity of oral LC. Data show that oral LC are more efficient than skin LC in providing co-stimulatory signals to T-cells, suggesting a difference in functional capacity between the two cell populations.

Key Words: epidermis • oral mucosa • mixed epithelial cell lymphocyte reaction • rats • T-cells

Journal of Dental Research, Vol. 78, No. 3, 751-758 (1999)
DOI: 10.1177/00220345990780030701


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