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Pellicle Precursor Proteins: Acidic Proline-rich Proteins, Statherin, and Histatins, and their Crosslinking Reaction by Oral Transglutaminase

Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, CABR W201, 700 Albany Street, Boston, Massachusetts 02118

M.S. Lamkin

Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, CABR W201, 700 Albany Street, Boston, Massachusetts 02118

EG Oppenheim

Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, CABR W201, 700 Albany Street, Boston, Massachusetts 02118, Department of Biochemistry, Boston University School of Medicine, 80 East Concord Street, Boston, Massachusetts 02118

Previous studies have demonstrated that whole saliva and pellicle formed in vitro from oral fluid contain covalently crosslinked salivary proteins. The purpose of this study was to determine which salivary proteins can act as substrates for transglutaminase, an enzyme responsible for the covalent crosslink reaction between a glutamine residue and a lysine residue. Transglutaminase was prepared from the pellet fraction of human whole saliva. Dansyl cadaverine (N-dansyl-1,5-diaminopentane) was used to study the reactivity of glutamine residues in acidic large and small proline-rich proteins, statherin, and the major histatins, whereas a glutamine-containing dansylated peptide was used to study the reactivity of lysine residues in these proteins. Crosslink formation was measured fluorometrically after the addition of fluorescent probe to the salivary protein substrate and transglutaminase. The covalent attachment of the fluorescent probe to salivary proteins was confirmed by SDS-PAGE. It was found that almost all of the lysines present in the acidic PRPs and statherin, and some of the lysines present in histatins, could participate in the crosslink reaction. Glutamine reactivity was also observed, but a maximum of only 14% of glutamine residues present in acidic PRPs and statherin participated in the crosslink formation. These results demonstrate that primary pellicle precursor proteins, acidic proline-rich proteins, statherin, and the major histatins are capable of undergoing crosslink reactions catalyzed by oral transglutaminase. This may enable other proteins in the oral cavity to be incorporated into the acquired enamel pellicle.

Key Words: salivary proteins • acquired enamel pellicle • transglutaminase

Journal of Dental Research, Vol. 78, No. 11, 1696-1703 (1999)
DOI: 10.1177/00220345990780110601


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