This Article Free Full Text (Free PDF) Free References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Murakami, S. Articles by Okada, H. Search for Related Content PubMed PubMed Citation Articles by Murakami, S. Articles by Okada, H. Social Bookmarking                         What's this?

Direct Interaction between Gingival Fibroblasts and Lymphoid Cells Induces Inflammatory Cytokine mRNA Expression in Gingival Fibroblasts

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

E. Hino

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

Y. Shimabukuro

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

T. Nozaki

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

Y. Kusumoto

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

T. Saho

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

F. Hirano

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

H. Hirano

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

H. Okada

Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka Suita, Osaka 565-0871, Japan

In inflamed periodontal lesions, dense infiltration of lymphocytes is usually observed in the extravascular periodontal connective tissue, adjacent to gingival fibroblasts. Our previous study revealed that activated lymphocytes can adhesively interact with gingival fibroblasts in vitro. In the present study, we investigated whether gingival fibroblasts are activated through direct interaction with lymphoid cells by monitoring the expression of inflammatory cytokine mRNA in human gingival fibroblasts (HGF). Co-culture with various human lymphoid cells in vitro resulted in a marked increase in the expression of IL-la, IL-1β, and IL-6 mRNA by the HGF. In addition, expression of the mRNA of the IL-1β-converting enzyme (ICE), which is essential to produce the mature form of IL-1β, was constitutively observed in the HGF, suggesting that mature IL-lp is produced by these cells. When HGF were cultured with the culture supernatant of the lymphoid cells, the increase in the inflammatory cytokine mRNA expression was not observed. Similarly, when HGF and lymphoid cells were cultured in the same well but separated by a membrane which prevented direct contact between the cells, no increase in inflammatory cytokine mRNA expression was observed. These results strongly indicate that direct interaction between these heterotypic cell types transduces activation signals into HGF that induce an increase in inflammatory cytokine mRNA expression. Furthermore, IL-1β mRNA expression in the HGF was synergistically increased when HGF directly interacted with lymphoid cells in the presence of exogeneous IL-1β. The present study demonstrates that direct interaction between HGF and lymphoid cells stimulates HGF to increase inflammatory cytokine mRNA expression, and raises the possibility that heterotypic cell-cell interaction may facilitate local inflammatory reactions.

Key Words: gingival fibroblast • lymphocyte • adhesive interaction • inflammatory cytokine • IL-1β

Journal of Dental Research, Vol. 78, No. 1, 69-76 (1999)
DOI: 10.1177/00220345990780011001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				P. Palmqvist, P. Lundberg, I. Lundgren, L. Hanstrom, and U.H. Lerner IL-1{beta} and TNF-{alpha} Regulate IL-6-type Cytokines in Gingival Fibroblasts Journal of Dental Research, June 1, 2008; 87(6): 558 - 563. [Abstract] [Full Text] [PDF]

				R. Mahanonda, N. Sa-Ard-Iam, P. Montreekachon, A. Pimkhaokham, K. Yongvanichit, M. M. Fukuda, and S. Pichyangkul IL-8 and IDO Expression by Human Gingival Fibroblasts via TLRs J. Immunol., January 15, 2007; 178(2): 1151 - 1157. [Abstract] [Full Text] [PDF]

				M.-E. Miranda-Carus, A. Balsa, M. Benito-Miguel, C. Perez de Ayala, and E. Martin-Mola IL-15 and the Initiation of Cell Contact-Dependent Synovial Fibroblast-T Lymphocyte Cross-Talk in Rheumatoid Arthritis: Effect of Methotrexate J. Immunol., July 15, 2004; 173(2): 1463 - 1476. [Abstract] [Full Text] [PDF]

				T. Kurita-Ochiai, K. Ochiai, N. Suzuki, K. Otsuka, and K. Fukushima Human Gingival Fibroblasts Rescue Butyric Acid-Induced T-Cell Apoptosis Infect. Immun., May 1, 2002; 70(5): 2361 - 2367. [Abstract] [Full Text] [PDF]

				S. Murakami, T. Hashikawa, T. Saho, M. Takedachi, T. Nozaki, Y. Shimabukuro, and H. Okada Adenosine regulates the IL-1{beta}-induced cellular functions of human gingival fibroblasts Int. Immunol., December 1, 2001; 13(12): 1533 - 1540. [Abstract] [Full Text] [PDF]

				M. Oido-Mori, R. Rezzonico, P.-L. Wang, Y. Kowashi, J.-M. Dayer, P. C. Baehni, and C. Chizzolini Porphyromonas gingivalis Gingipain-R Enhances Interleukin-8 but Decreases Gamma Interferon-Inducible Protein 10 Production by Human Gingival Fibroblasts in Response to T-Cell Contact Infect. Immun., July 1, 2001; 69(7): 4493 - 4501. [Abstract] [Full Text] [PDF]