This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Okamoto, K. Articles by Akagawa, Y. Search for Related Content PubMed PubMed Citation Articles by Okamoto, K. Articles by Akagawa, Y. Social Bookmarking                         What's this?

RGD Peptides Regulate the Specific Adhesion Scheme of Osteoblasts to Hydroxyapatite but not to Titanium

Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan

T. Matsuura

Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan

R. Hosokawa

Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan

Y. Akagawa

Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan

Hydroxyapatite (HA) is a bioactive dental implant material which accelerates bone formation on its surface. The mechanism of this acceleration is not clear. The elucidation of the cell adhesion might be the key to the understanding of the bioactive mechanism of HA. In this study, we analyzed the adhesion of HOS human osteoblasts onto HA and titanium to find the particular adhesion to HA. In short-term cultures in fetal bovine serum-pre-coated materials, a significantly higher number of cells adhered to HA than to titanium. In addition, serum-free conditions with phosphate-buffered saline pre-coating or bovine serum albumin pre-coating materials were tested. The results were nearly the same among all pre-coating conditions, suggesting that the quantity of cell adhesion was not affected by serum components. However, in the morphological observations by SEM, the form of adhesion was found to differ among pre-coating conditions. The osteoblasts tightly adhered and spread onto both HA and titanium with serum pre-coating, whereas the cells loosely adhered and did not spread without serum. To evaluate the Arg-Gly-Asp (RGD) sequence-specific adhesion, we used synthetic RGD peptides for a competitive inhibition test. The results showed that RGD peptides remarkably inhibited the tight adhesion and spreading of osteoblasts onto HA, whereas they did not strongly inhibit adhesion and spreading onto titanium. These results demonstrate that the regulation of cell adhesion to HA is different from that to titanium. Our study suggests that the RGD-containing serum proteins might have a major role in regulating the specific adhesion of osteoblasts to HA, and in inducing enhanced cell growth and differentiation.

Key Words: RGD peptide • adhesion • osteoblast • hydroxyapatite • titanium.

Journal of Dental Research, Vol. 77, No. 3, 481-487 (1998)
DOI: 10.1177/00220345980770030701


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				B. ALLAN Closer to nature: new biomaterials and tissue engineering in ophthalmology Br J Ophthalmol, November 1, 1999; 83(11): 1235 - 1240. [Full Text]