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Porphyromonas gingivalis Fimbriae Mediate Coaggregation with Streptococcus oralis through Specific Domains

Department of Preventive Dentistry

T. Fujiwara

Department of Pediatric Dentistry

H. Nagata

Department of Preventive Dentistry, Department of Oral Biology, School of Dental Medicine

M. Kuboniwa

Department of Preventive Dentistry

A. Sharma

Department of Oral Biology, School of Dental Medicine

H.T. Sojar

Department of Oral Biology, School of Dental Medicine

R.J. Genco

Department of Oral Biology, School of Dental Medicine, Department of Microbiology, School of Medicine and Biomedical Science, State University of New York at Buffalo, 3435 Main Street, Buffalo, New York 14214, USA

S. Hamada

Department of Oral Microbiology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565, Japan

S. Shizukuishi

Department of Preventive Dentistry

Fimbriae are major adhesive components on the cell surface of Porphyromonas gingivalis. In this study, we evaluated the role of fimbriae in coaggregation with Streptococcus oralis. Fimbriae purified from P. gingivalis competitively inhibited the coaggregation by 100% at a concentration of 50 ug/mL. On the other hand, the same amount of lipopolysaccharide isolated from P. gingivalis was inhibited by only 25% of the level of the fimbriae. A fimA-inactivated mutant of P. gingivalis failed to show distinct coaggregation activity. Fimbriae added to a solution of various strains of streptococci caused their self-aggregation at a concentration of 10 to 30 ug/mL. The self-aggregation induced by fimbriae was inhibited by \-arginine (20 to 40 mM/L). Iodinated fimbriae reacted with S. oralis cells immobilized on the nitrocellulose membrane, and 100°C heating of the cells diminished the binding abilities. Recombinant fimbrillin (r-Fim, corresponding to whole residues 1 to 337 of native fimbrillin) of P. gingivalis also showed 100% inhibition of the coaggregation. The r-Fim variant (residues 1 to 286) lacking the C-terminal 51 residues was as inhibitory as r-Fim. However, the variant (residues 1 to 265) without the C-terminal 72 residues lost 77% of the inhibitory activity. These findings suggested that residues 266 to 286 contain a domain involved in the coaggregation of P. gingivalis with S. oral is. Inhibition by three polypeptides corresponding to residues 266 to 286, 266 to 337, and 287 to 337 was studied. Peptides 266 to 286 and 266 to 337 inhibited by 96 and 100%, respectively, at a concentration of 1.5 nmol/mL. Peptide 287 to 337 also showed a significant inhibitory effect but to a slightly lesser extent than that of peptide 266 to 286. P. gingivalis fimbriae appear to be involved in coaggregation with streptococci, probably through an adhesive protein molecule(s) of the latter, and the fimbriae possess several domains in the C-terminal residues 266 to 337 for interaction with S. oralis.

Key Words: P. gingival is • S. oral is • fimbriae • coaggregation • binding domain.

Journal of Dental Research, Vol. 76, No. 4, 852-857 (1997)
DOI: 10.1177/00220345970760040601


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