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Structural and Functional Association between Substance P- and Calcitonin Gene-related Peptide-immunoreactive Nerves and Accessory Cells in the Rat Dental Pulp

Department of Endodontology and Oral Diagnosis, Faculty of Odontology, Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-chome, Bunkyo-ku, Tokyo 113, Japan

M. Jontell

Department of Endodontology and Oral Diagnosis, Faculty of Odontology

P. Belichenko

Institute of Neurobiology, Department of Anatomy and Cell Biology

U. Dahlgren

Department of Endodontology and Oral Diagnosis, Faculty of Odontology, Department of Clinical Immunology, Goteborg University, Medicinaregatan 12, S-413 90 Goteborg, Sweden

G. Bergenholtz

Department of Endodontology and Oral Diagnosis, Faculty of Odontology

A. Dahlstrom

Institute of Neurobiology, Department of Anatomy and Cell Biology

Defense mechanisms of the dentin/pulp complex involve a variety of biological systems in which immunocompetent cells, the nervous system, and the vascular supply play important roles. In the present study, pulpal accessory cells were examined regarding (i) their structural relationship to nerves and (ii) how the functional capacities of these cells were affected by neuropeptides. Micro-anatomic association was investigated in the normal rat molar pulp with the use of double-immunofluorescence staining and dual-channel confocal laser scanning microscopy. Examinations of confocal laser scanning microscopic images from single focal planes revealed the presence of apparent contacts between thin, varicose nerve fibers and immunocompetent cells, indicating proximity between these two structures. The close associations were most frequently observed in the para-odontoblastic region of the coronal pulp, where more than 70% of class II antigen-expressing (OX6+) cells showed proximity to nerve fibers immunoreactive to calcitonin gene-related peptide. The corresponding figure for substance P was about 50%. ED2+ macrophages closely associated with nerves were less frequently observed. Functional studies conducted in vitro demonstrated that 10-9 to 10-7 mol/L of substance P significantly increased (p < 0.05), while 10-7 to 10-6 mol/L of calcitonin gene-related peptide suppressed (p < 0.01) proliferation of purified T-lymphocytes stimulated with sub-optimal concentrations of concanavalin A in the presence of rat incisor pulpal cells as accessory cells. These data suggest that pulpal sensory nerve fibers and their products may have an influence upon the immune defense of the dental pulp.

Key Words: Class II antigen-expressing cells • pulpal innervation • mitogen-induced T-lymphocyte proliferation.

Journal of Dental Research, Vol. 76, No. 12, 1818-1824 (1997)
DOI: 10.1177/00220345970760120301


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