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Stimulation of Platelet-activating Factor Synthesis by Neurotransmitters in Salivary GlandsDepartment of Pharmacology
Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan
Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan
Department of Removable Prosthodontics, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima 734, Japan
Department of Pharmacology
Department of Pharmacology
Platelet-activating factor (PAF), a phospholipid mediator exhibiting potent biological activities, has been shown to stimulate amylase release from the pancreas and salivary glands. The capacity of salivary glands for PAF biosynthesis in response to stimulation has also been demonstrated. To elucidate the role of PAF in salivary glands, we studied the regulation of platelet-activating factor synthesis by the autonomic nervous system in canine salivary glands. Acetylcholine and ionomycin stimulated PAF production in dispersed cells from parotid, submandibular, and sublingual glands of dogs. Norepinephrine and phenylephrine, but not isoproterenol, also stimulated PAF production in submandibular gland cells. Norepinephrine-induced PAF production was blocked by phentolamine but not by propranolol. Acetylcholine and norepinephrine increased both the PAF production and liberation of [14C]arachidonic acid from cells pre-labeled with [14C]arachidonic acid in the presence of Ca2+ in the medium. These stimulants increased [ 14C]arachidonic acid liberation without the accompanying production of PAF in Ca2+-deprived medium. No activators or inhibitors of protein kinase C produced or affected acetylcholine-induced PAF production. Lyso-PAF:acetyl-CoA acetyltransferase was activated in the cells treated with acetylcholine, norepinephrine, isoproterenol, and 8Br-cyclic AMP. Deprivation of Ca2+ in the medium markedly reduced acetylcholine-induced activation of the transferase, but little affected norepinephrine-, isoproterenol-, and 8Br-cyclic AMP-induced activation. Dithiothreitol-insensitive cholinephosphotransferase activity was also increased by acetylcholine, norepinephrine, isoproterenol, and 8Br-cyclic AMP, and the deprivation of Ca2+ in the medium further increased the activation of the enzyme activity by these agents. These results suggest that PAF synthesis in canine salivary glands is under the control of muscarinic cholinergic and
Key Words: PAF • salivary gland • acetylcholine • norepinephrine.
Journal of Dental Research, Vol. 76, No. 1,
568-574 (1997) This article has been cited by other articles:
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-adrenergic systems via Ca2+-dependent remodeling pathways, and that the independent activation of either phospholipase A2 or acetyltransferase is insufficient for PAF production in submandibular gland cells, i.e., the concurrent activation of these enzymes is required. 
