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Journal of Dental Research
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Electrophoretic Gels of Dentin Matrix Proteins as Diffusion Media for in vitro Mineralization

Y. Wada

Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan 060

R. Fujisawa

Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan 060

Y. Nodasaka

Laboratory of Electron Microscopy, School of Dentistry, Hokkaido University, Sapporo, Japan 060

Y. Kuboki

Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan 060

Non-collagenous proteins of dentin and bone have important effects on mineralization which have been studied by various in vitro systems. We developed an in vitro mineralization system using electrophoretic gels as diffusion media of calcium and phosphate ions. Calcium and phosphate ions were diffused naturally or propelled by electric potential. Calcium phosphate was precipitated in the gel, and the precipitation was affected by proteins in the gel which had been separated by electrophoresis. We applied this system to analysis of non-collagenous proteins of dentin. Among the proteins, phosphophoryns promoted calcium phosphate precipitation in the natural-diffusion system. A non-collagenous protein having a molecular mass of 60 kDa inhibited precipitation. The results were different, however, in the electric-diffusion system, in which phosphophoryns had a negative effect. The present system enabled us to compare the effects of plural proteins rapidly, even using unpurified material.

Journal of Dental Research, Vol. 75, No. 6, 1381-1387 (1996)
DOI: 10.1177/00220345960750060901
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