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Identification of the Fas Antigen in Human Gingiva

First Department of Oral Surgery, Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

Y. Muraki

First Department of Oral Surgery , Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

J. Fukuda

First Department of Oral Surgery, Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

T. Haneji

First Department of Oral Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

N. Kobayashi

Department of Virology and Parasitology, Yamaguchi University, School of Medicine, Kogushi, Ube, Yamaguchi 755, Japan

The Fas antigen is a cell-surface glycoprotein that mediates apoptosis from the cell surface into the cytoplasm. Polyclonal antibody (Fas D) was raised against a synthetic polypeptide selected from the extracellular part of the human Fas antigen (amino acid residues 104-114) and was used to detect the Fas antigen in human gingiva. Biopsy specimens of human gingiva were prepared, and the paraffin sections were reacted with the Fas D antibody by an immunohistochemical method. The antibody localized to the prickle-cell layer and to granular layer keratinocytes of human gingiva. Proteins were also prepared from human gingiva and subjected to SDS-PAGE, followed by Western-blotting analysis with the Fas D antibody. The antibody interacted with a band corresponding to an estimated molecular weight of 35 kDa. The incidence of the immunoreactive 35-kDa protein was detected in the gingiva of 90% of the 20 individuals examined. The Fas antigen detected in human gingiva may be related to the physiological turnover of oral mucosa.

Key Words: Fas antigen • human gingiva • apoptosis

Journal of Dental Research, Vol. 75, No. 6, 1353-1357 (1996)
DOI: 10.1177/00220345960750060501


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