|
Sign In to gain access to subscriptions and/or personal tools.
|
Differential Expression of Human High-molecular-weight Salivary Mucin (MG1) and Low-molecular-weight Salivary Mucin (MG2)
P.A. Nielsen
Department of Oral Diagnostics, Faculty of Health Sciences, School of Dentistry, University of Copenhagen, Norre Allé 20, 2200 Copenhagen N, Denmark
U. Mandel
Department of Oral Diagnostics, Faculty of Health Sciences, School of Dentistry, University of Copenhagen, Norre Allé 20, 2200 Copenhagen N, Denmark
M.H. Therkildsen
Department of Pathology, Rigshospitalet, Copenhagen, Denmark
H. Clausen
Department of Oral Diagnostics, Faculty of Health Sciences, School of Dentistry, University of Copenhagen, Norre Allé 20, 2200 Copenhagen N, Denmark
Two distinct mucin components of saliva, MG1 and MG2, have been identified based on chemical composition and molecular weights (high and low, respectively) in saliva. With the aim of characterizing the expression pattern of salivary mucins, we have prepared monoclonal antibodies (MAbs) directed against the peptide core of MG1 and against a synthetic peptide derived from the MG2 (MUC7) sequence. MAb PANH2 raised against partially deglycosylated MG1 stained a high-molecular-weight smear in Western blots of partially purified MG1. PANH2 binding was increased by deglycosylation with trifluoromethanesulfonic acid as well as with subsequent periodate treatment, and was eliminated by pronase treatment, strongly suggesting that MAb PANH2 was directed to a peptide epitope of MG1. MAb PANH3 raised against a synthetic peptide derived from the MG2 (MUC7) sequence reacted with the native molecule and stained a narrow smear of ca. 200,000 to 210,000 in Western blots of concentrated saliva and a lower-molecular-weight smear of trifluoromethanesulfonic-acid-treated MG2. Immunohistology on frozen sections of human salivary glands showed that MAb PANH2 selectively labeled mucous cells, whereas MAb PANH3 labeled subpopulations of serous cells. Double-direct immunofluorescence staining with PANH2 and PANH3 demonstrated that the staining patterns were non-overlapping. The development of these antibody probes will facilitate studies of mucin expression in diseases of salivary glands.
Key Words: mucins, • MUC7, • MG1 • monoclonal antibodies • salivary gland
Journal of Dental Research, Vol. 75, No. 11,
1820-1826 (1996)
DOI: 10.1177/00220345960750110201
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
N. G Karlsson and K. A Thomsson
Salivary MUC7 is a major carrier of blood group I type O-linked oligosaccharides serving as the scaffold for sialyl Lewis x
Glycobiology,
March 1, 2009;
19(3):
288 - 300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C Alliende, Y-J Kwon, M Brito, C Molina, S Aguilera, P Perez, L Leyton, A F G Quest, U Mandel, E Veerman, et al.
Reduced sulfation of muc5b is linked to xerostomia in patients with Sjogren syndrome
Ann Rheum Dis,
October 1, 2008;
67(10):
1480 - 1487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Thomsson, B. L. Schulz, N. H. Packer, and N. G. Karlsson
MUC5B glycosylation in human saliva reflects blood group and secretor status
Glycobiology,
August 1, 2005;
15(8):
791 - 804.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Piludu, S. A. Rayment, B. Liu, G. D. Offner, F. G. Oppenheim, R. F. Troxler, and A. R. Hand
Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands
J. Histochem. Cytochem.,
January 1, 2003;
51(1):
69 - 79.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. P. Paulsen, M. Hinz, U. Schaudig, A. B. Thale, and W. Hoffmann
TFF Peptides in the Human Efferent Tear Ducts
Invest. Ophthalmol. Vis. Sci.,
November 1, 2002;
43(11):
3359 - 3364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Liu, J. R. Lague, D. P. Nunes, P. Toselli, F. G. Oppenheim, R. V. Soares, R. F. Troxler, and G. D. Offner
Expression of Membrane-associated Mucins MUC1 and MUC4 in Major Human Salivary Glands
J. Histochem. Cytochem.,
June 1, 2002;
50(6):
811 - 820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Thomsson, A. Prakobphol, H. Leffler, M. S. Reddy, M. J. Levine, S. J. Fisher, and G. C. Hansson
The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse
Glycobiology,
January 1, 2002;
12(1):
1 - 14.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.G.M. Bolscher, J. Groenink, J.S. van der Kwaak, P.A.M. van den Keijbus, W. van't Hof, E.C.I. Veerman, and A.V. Nieuw Amerongen
Detection and Quantification of MUC7 in Submandibular, Sublingual, Palatine, and Labial Saliva by Anti-peptide Antiserum
Journal of Dental Research,
July 1, 1999;
78(7):
1362 - 1369.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. A. Reis, L. David, P. Correa, F. Carneiro, C. d. Bolos, E. Garcia, U. Mandel, H. Clausen, and M. Sobrinho-Simoes
Intestinal Metaplasia of Human Stomach Displays Distinct Patterns of Mucin (MUC1, MUC2, MUC5AC, and MUC6) Expression
Cancer Res.,
March 1, 1999;
59(5):
1003 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. P. Bennett, H. Hassan, U. Mandel, E. Mirgorodskaya, P. Roepstorff, J. Burchell, J. Taylor-Papadimitriou, M. A. Hollingsworth, G. Merkx, A. G. van Kessel, et al.
Cloning of a Human UDP-N-Acetyl-alpha -D-Galactosamine:Polypeptide N-Acetylgalactosaminyltransferase That Complements Other GalNAc-Transferases in Complete O-Glycosylation of the MUC1 Tandem Repeat
J. Biol. Chem.,
November 13, 1998;
273(46):
30472 - 30481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Sharma, L. Dudus, P. A. Nielsen, H. Clausen, J. R. Yankaskas, M. A. Hollingsworth, and J. F. Engelhardt
MUC5B and MUC7 Are Differentially Expressed in Mucous and Serous Cells of Submucosal Glands in Human Bronchial Airways
Am. J. Respir. Cell Mol. Biol.,
July 1, 1998;
19(1):
30 - 37.
[Abstract]
[Full Text]
|
|
|
|
|
