This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Wise, G.E. Articles by Zhao, L. Search for Related Content PubMed PubMed Citation Articles by Wise, G.E. Articles by Zhao, L. Social Bookmarking                         What's this?

Transcription and Translation of CSF-1 in the Dental Follicle

Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803-8408

F. Lin

Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803-8408

L. Zhao

Department of Veterinary Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803-8408

The dental follicle, a loose connective tissue sac which surrounds the unerupted tooth, is required for eruption to occur. Injection of colony-stimulating factor-1 (CSF-1) will accelerate molar eruption in rats, as well as stimulate tooth eruption in osteopetrotic rats. Utilizing in situ hybridization and reverse- transcription polymerase chain-reaction techniques, we show here that CSF-1 mRNA is present in vivo in the dental follicle of the first mandibular molar of the rat. Analysis of the molars from day 0 through day 10 post-natally demonstrates that the maximal expression of CSF-1 mRNA is at day 3 post-natally. Immunostaining also reveals that the CSF-1 mRNA is translated, with immunostaining for the CSF-1 itself, being heavy in early post-natal days and absent by day 9 post-natally. In view of the fact that there is a maximal influx of mononuclear cells (monocytes) into the dental follicle at day 3 post-natally-an influx which increases the numbers of osteoclasts needed to form a tooth eruption pathway-it is probable that the maximal expression of CSF-1 mRNA by day 3 post-natally contributes to this monocyte influx. Thus, this study establishes a relationship among a molecule (CSF-1), cell (monocyte), and tissue (dental follicle) that appear to play a major role in tooth eruption.

Key Words: CSF-1 mRNA • CSF-1 • dental follicle • tooth eruption.

Journal of Dental Research, Vol. 74, No. 9, 1551-1557 (1995)
DOI: 10.1177/00220345950740090801


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				G.E. Wise, S. Frazier-Bowers, and R.N. D'Souza CELLULAR, MOLECULAR, AND GENETIC DETERMINANTS OF TOOTH ERUPTION Critical Reviews in Oral Biology & Medicine, July 1, 2002; 13(4): 323 - 335. [Abstract] [Full Text] [PDF]

				G.E. Wise The Biology of Tooth Eruption Journal of Dental Research, August 1, 1998; 77(8): 1576 - 1579. [PDF]

				R.L. Grier IV and G.E. Wise Inhibition of Tooth Eruption in the Rat by a Bisphosphonate Journal of Dental Research, January 1, 1998; 77(1): 8 - 15. [Abstract] [PDF]

				G.E. Wise, L. Zhao, and R.L. Grier IV Localization and Expression of CSF-1 Receptor in Rat Dental Follicle Cells Journal of Dental Research, June 1, 1997; 76(6): 1244 - 1249. [Abstract] [PDF]

				I. A. Nakchbandi, E. E. Weir, K. L. Insogna, W. M. Philbrick, and A. E. Broadus Parathyroid hormone-related protein induces spontaneous osteoclast formation via a paracrine cascade PNAS, June 20, 2000; 97(13): 7296 - 7300. [Abstract] [Full Text] [PDF]