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Effects of Dopamine on Adenylyl Cyclase Activity and Amylase Secretion in Rat Parotid Tissue

Department of Pharmacology, School of Medicine, Sapporo Medical University, South-1, West-17, Chuo-ku, Sapporo 060, Japan

N. Amemiya

Department of Pharmacology, School of Medicine, Sapporo Medical University, South-1, West-17, Chuo-ku, Sapporo 060, Japan

H. Takemura

Department of Pharmacology, School of Medicine, Sapporo Medical University, South-1, West-17, Chuo-ku, Sapporo 060, Japan

H. Ohshika

Department of Pharmacology, School of Medicine, Sapporo Medical University, South-1, West-17, Chuo-ku, Sapporo 060, Japan

Several previous studies have shown that dopamine causes amylase secretion from rat parotid tissue. However, the mechanism of this dopamine action is still unclear. The present study was designed to characterize dopamine action in rat parotid gland tissue by examining the effects of dopamine on cyclic AMP accumulation, adenylyl cyclase activity, and amylase release. Dopamine significantly enhanced accumulation of cyclic AMP in parotid slices and stimulated adenylyl cyclase activity in parotid membrane preparations. It also significantly stimulated amylase release from parotid slices. The stimulatory effects of dopamine on cyclic AMP accumulation, adenylyl cyclase activity, and amylase release were effectively blocked with propranolol, a β-adrenergic antagonist, but not by either SCH 23390, a preferential D₁ antagonist, or butaclamol, a preferential D2 antagonist. No substantial specific binding sites for D, receptors were detectable by [³H]SCH 23390 binding in parotid membranes. These results suggest that the stimulatory effect of dopamine on amylase secretion in rat parotid tissue is not mediated through specific D₁ dopamine receptors but rather through β-adrenergic receptors.

Key Words: dopamine receptor • β-adrenergic receptor • parotid gland • rat

Journal of Dental Research, Vol. 74, No. 6, 1289-1294 (1995)
DOI: 10.1177/00220345950740060801


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