Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Free Full Text (Free PDF) Free
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Quintero, J.C.
Right arrow Articles by Agarwal, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quintero, J.C.
Right arrow Articles by Agarwal, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

LPS Responsiveness in Periodontal Ligament Cells is Regulated by Tumor Necrosis Factor-{alpha}

J.C. Quintero

Division of Oral Biology, 589 Salk Hall, University of Pittsburgh, Pittsburgh, PA 15261

N.P. Piesco

Division of Oral Biology, 589 Salk Hall, University of Pittsburgh, Pittsburgh, PA 15261

H.H. Langkamp

Division of Oral Biology, 589 Salk Hall, University of Pittsburgh, Pittsburgh, PA 15261

L. Bowen

Division of Dental Surgical Sciences, 589 Salk Hall, University of Pittsburgh, Pittsburgh, PA 15261

S. Agarwal

Division of Oral Biology, 589 Salk Hall, University of Pittsburgh, Pittsburgh, PA 15261

Gingival fibroblasts function as accessory immune cells and are capable of synthesizing cytokines in response to lipopolysaccharides (LPS) from Gram-negative microbes. Recently, we have isolated, cloned, and characterized two cell lines which exhibit characteristics of periodontal ligament (PDL) cells. In this report, we demonstrate that PDL cells showing osteoblast-like phenotype are not LPSresponsive cells. However, treatment of PDL cells with tumor necrosis factor-{alpha} (TNF-a) inhibits the expression of their osteoblast-like characteristics. As a consequence of this TNF-a-induced phenotypic change, PDL cells become LPSresponsive, i.e., synthesize several pro-inflammatory cytokines in response to LPS. These phenotypic changes occur at concentrations of TNF-a that are frequently observed in tissue exudates during periodontal inflammation, suggesting a physiological significance for these in vitro observations. It is of interest that TNF-a-induced phenotypic changes in PDL cells are transient, since removal of rhTNF-a from the supernatants of PDL cell cultures results in re-acquisition of the osteoblast-like characteristics and lack of LPS responsiveness of PDL cells. These results suggest that TNF-a, by regulating the PDL cell functions, may allow these cells to participate in the disease process as accessory immune cells at the expense of their structural properties.

Key Words: periodontal ligament cells • lipopolysaccharide • tumor necrosis factor-{alpha}

Journal of Dental Research, Vol. 74, No. 11, 1802-1811 (1995)
DOI: 10.1177/00220345950740111401
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
M. Kobayashi, T. Takiguchi, R. Suzuki, A. Yamaguchi, K. Deguchi, M. Shionome, Y. Miyazawa, T. Nishihara, M. Nagumo, and K. Hasegawa
Recombinant Human Bone Morphogenetic Protein-2 Stimulates Osteoblastic Differentiation in Cells Isolated from Human Periodontal Ligament
Journal of Dental Research, October 1, 1999; 78(10): 1624 - 1633.
[Abstract] [PDF]

Home page
 JDRHome page
T.A. Brady, N.P. Piesco, M.J. Buckley, H.H. Langkamp, L.L. Bowen, and S. Agarwal
Autoregulation of Periodontal Ligament Cell Phenotype and Functions by Transforming Growth Factor-{beta}1
Journal of Dental Research, October 1, 1998; 77(10): 1779 - 1790.
[Abstract] [PDF]

Home page
 Infect. Immun.Home page
S. Agarwal, C. S. Chandra, N. P. Piesco, H. H. Langkamp, L. Bowen, and C. Baran
Regulation of Periodontal Ligament Cell Functions by Interleukin-1beta
Infect. Immun., March 1, 1998; 66(3): 932 - 937.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
P.C. Lekic, N. Pender, and C.A.G. McCulloch
Is Fibroblast Heterogeneity Relevant To the Health, Diseases, and Treatments of Periodontal Tissues?
Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(3): 253 - 268.
[Abstract] [Full Text] [PDF]