This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Tjäderhane, L. Articles by Larmas, M. Search for Related Content PubMed PubMed Citation Articles by Tjäderhane, L. Articles by Larmas, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL MAGNESIUM COMPOUNDS MAGNESIUM, ELEMENTAL PHOSPHORUS SODIUM ZINC COMPOUNDS ZINC, ELEMENTAL Social Bookmarking                         What's this?

Mineral Element Analysis of Carious and Sound Rat Dentin by Electron Probe Microanalyzer Combined with Back-scattered Electron Image

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland

E.-L. Hietala

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland

M. Larmas

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland, Oulu University Hospital

We recently demonstrated the advantages of back-scattered electron images (COMPO) in the visualization of dentinal caries, and the relationship of the change in the dentin fluorescence pattern in caries lesions. However, the exact nature of these changes is not known. In this paper, the nature of the changes in the areas with reduced mineral content in COMPO images was investigated. We examined the relation of changes in mineral elements and the appearance of soft carious and sound dentin in COMPO images using a scanning electron microscope (SEM) equipped with an electron probe microanalyzer (EPMA). Rat molars with small dentinal caries lesions just under the DEJ were chosen for the study. The Ca, P, Na, Mg, Zn, F, and total contents were determined by EPMA from five different dentin sites, and the Ca/P and Mg/Ca ratios were calculated. Generally, the lowest contents were found in caries lesions and highest in mantle dentin, with the exceptions of Mg and Zn. The Ca/P ratio was lowest in mantle dentin and highest in carious dentin. The results confirm that the change in fluorescence in the dentinal caries lesion is correlated with the very initial changes in mineral content, and that EPMA used in combination with COMPO images is a useful tool for determining small changes in mineral elements in the carious and adjacent areas of dentin.

Key Words: dentin • rat • normal • carious • mineral elements

Journal of Dental Research, Vol. 74, No. 11, 1770-1774 (1995)
DOI: 10.1177/00220345950740110901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				L. Valikangas, E. Pekkala, M. Larmas, J. Risteli, T. Salo, and L. Tjaderhane The Effects of High levels of Glucose and Insulin on Type I Collagen Synthesis in Mature Human Odontoblasts and Pulp Tissue in vitro Advances in Dental Research, August 1, 2001; 15(1): 72 - 75. [Abstract] [PDF]