This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Matsuki, Y. Articles by Yamada, Y. Search for Related Content PubMed PubMed Citation Articles by Matsuki, Y. Articles by Yamada, Y. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein Social Bookmarking                         What's this?

A Compilation of Partial Sequences of Randomly Selected cDNA Clones from the Rat Incisor

Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892

M. Nakashima

Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892

N. Amizukal

Department of Anatomy and Cell Biology, McGill University, Montreal QC H3A 1A1, Canada, Calcium Research Laboratory, Royal Victoria Hospital, McGill University, Montreal QC H3A 1A1, Canada, Department of Medicine, McGill University, Montreal QC H3A 1A1, Canada

H. Warshawsky

Department of Anatomy and Cell Biology, McGill University, Montreal QC H3A 1A1, Canada

D. Goltzman

Calcium Research Laboratory, Royal Victoria Hospital, McGill University, Montreal QC H3A 1A1, Canada, Department of Medicine, McGill University, Montreal QC H3A 1A1, Canada

K.M. Yamada

Laboratory of Developmental Biology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892

Y. Yamada

The formation of tooth organs is regulated by a series of developmental programs. We have initiated a genome project with the ultimate goal of identifying novel genes important for tooth development. As an initial approach, we constructed a unidirectional cDNA library from the non-calcified portion of incisors of 3- to 4-week-old rats, sequenced cDNA clones, and classified their sequences by homology search through the GenBank data base and the PIR protein data base. Here, we report partial DNA sequences obtained by automated DNA sequencing on 400 cDNA clones randomly selected from the library. Of the sequences determined, 51% represented sequences of new genes that were not related to any previously reported gene. Twenty-six percent of the clones strongly matched genes and proteins in the data bases, including amelogenin, 1(I) and a2(I) collagen chains, osteonectin, and decorin. Nine percent of clones revealed partial sequence homology to known genes such as transcription factors and cell surface receptors. A significant number of the previously identified genes were expressed redundantly and were found to encode extracellular matrix proteins. Identification and cataloging of cDNA clones in these tissues are the first step toward identification of markers expressed in a tissue- or stage-specific manner, as well as the genetic linkage study of tooth anomalies. Further characterization of the clones described in this paper should lead to the discovery of novel genes important for tooth development.

Key Words: gene expression • cDNA library • sequence • rat incisor

Journal of Dental Research, Vol. 74, No. 1, 307-312 (1995)
DOI: 10.1177/00220345950740010401


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R. A. Wagenaar-Miller, L. H. Engelholm, J. Gavard, S. S. Yamada, J. S. Gutkind, N. Behrendt, T. H. Bugge, and K. Holmbeck Complementary Roles of Intracellular and Pericellular Collagen Degradation Pathways In Vivo Mol. Cell. Biol., September 15, 2007; 27(18): 6309 - 6322. [Abstract] [Full Text] [PDF]

				B. Ganss and A. Jheon ZINC FINGER TRANSCRIPTION FACTORS IN SKELETAL DEVELOPMENT Critical Reviews in Oral Biology & Medicine, September 1, 2004; 15(5): 282 - 297. [Abstract] [Full Text] [PDF]

				M. Lin, D. R. Sutherland, W. Horsfall, N. Totty, E. Yeo, R. Nayar, X.-F. Wu, and A. C. Schuh Cell surface antigen CD109 is a novel member of the alpha 2 macroglobulin/C3, C4, C5 family of thioester-containing proteins Blood, March 1, 2002; 99(5): 1683 - 1691. [Abstract] [Full Text] [PDF]

				A. Nanci, P. Mocetti, Y. Sakamoto, M. Kunikata, E. Lozupone, and E. Bonucci Morphological and Immunocytochemical Analyses on the Effects of Diet-induced Hypocalcemia on Enamel Maturation in the Rat Incisor J. Histochem. Cytochem., August 1, 2000; 48(8): 1043 - 1058. [Abstract] [Full Text]

				Y. Liu, H. Li, K. Tanaka, N. Tsumaki, and Y. Yamada Identification of an Enhancer Sequence within the First Intron Required for Cartilage-specific Transcription of the alpha 2(XI) Collagen Gene J. Biol. Chem., April 21, 2000; 275(17): 12712 - 12718. [Abstract] [Full Text] [PDF]

				A. Nanci, S. Zalzal, P. Lavoie, M. Kunikata, W.-Y. Chen, P.H. Krebsbach, Y. Yamada, L. Hammarström, J.P. Simmer, A.G. Fincham, et al. Comparative Immunochemical Analyses of the Developmental Expression and Distribution of Ameloblastin and Amelogenin in Rat Incisors J. Histochem. Cytochem., August 1, 1998; 46(8): 911 - 934. [Abstract] [Full Text]

				T. Uchida, C. Murakami, N. Dohi, K. Wakida, T. Satoda, and O. Takahashi Synthesis, Secretion, Degradation, and Fate of Ameloblastin During the Matrix Formation Stage of the Rat Incisor as Shown by Immunocytochemistry and Immunochemistry Using Region-specific Antibodies J. Histochem. Cytochem., October 1, 1997; 45(10): 1329 - 1340. [Abstract] [Full Text] [PDF]

				T. Toyono, M. Nakashima, S. Kuhara, and A. Akamine Expression of TGF-{beta} Superfamily Receptors in Dental Pulp Journal of Dental Research, September 1, 1997; 76(9): 1555 - 1560. [Abstract] [PDF]

				M. MacDougall, D. Simmons, X. Luan, J. Nydegger, J. Feng, and T. T. Gu Dentin Phosphoprotein and Dentin Sialoprotein Are Cleavage Products Expressed from a Single Transcript Coded by a Gene on Human Chromosome 4. DENTIN PHOSPHOPROTEIN DNA SEQUENCE DETERMINATION J. Biol. Chem., January 10, 1997; 272(2): 835 - 842. [Abstract] [Full Text] [PDF]

				S. M. King, E. Barbarese, J. F. Dillman III, R. S. Patel-King, J. H. Carson, and K. K. Pfister Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000Light Chain J. Biol. Chem., August 9, 1996; 271(32): 19358 - 19366. [Abstract] [Full Text] [PDF]

				P. H. Krebsbach, S. K. Lee, Y. Matsuki, C. A. Kozak, K. M. Yamada, and Y. Yamada Full-length Sequence, Localization, and Chromosomal Mapping of Ameloblastin J. Biol. Chem., February 23, 1996; 271(8): 4431 - 4435. [Abstract] [Full Text] [PDF]