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Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 W. 168th St., New York, New York 10032

G. Bhargava

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 W. 168th St., New York, New York 10032

F. Nishimura

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 W. 168th St., New York, New York 10032

R. Orman

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 W. 168th St., New York, New York 10032

R. Price

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 W. 168th St., New York, New York 10032

V.P. Terranova

Bronx VAMC. 130 W. Kingsbridge Rd., Bronx, New York 10468

A major focus of studies that center on regeneration of the periodontium is to determine the efficacy of the use of polypeptide growth factors. Platelet-derived growth factor has been reported to be a possible agent for clinical use. PDGF has various isoforms. Therefore, we decided to study the mitogenic and chemotactic responses of human periodontal ligament (PDL) cells to recombinant human PDGF-AB, AA, and BB. Addition of each isoform of PDGF to in vitro mitogenesis assays induced PDL cell proliferation in a dose-dependent manner. The maximum mitogenic effect was evident at the concentration of 100 ng/mL. In these assays, PDGF-BB was found to be the most potent mitogen. PDGF-AB elicited an intermediate response, and PDGF-AA was the least effective. The results of chemotaxis assays closely parallel those of the mitogenesis assays. PDGF-BB exhibited the most potent chemotactic effect. The maximal effect was observed at 10 ng/mL. The findings of these experiments indicate that PDGF-BB is more effective than the other isoforms in promoting mitogenesis and chemotaxis of PDL cells in vitro, and may therefore be a suitable ethical pharmaceutical for use in periodontal regeneration procedures.

Key Words: Periodontal Ligament • Growth Factors • Chemotaxis • Proliferation.

Journal of Dental Research, Vol. 73, No. 10, 1593-1600 (1994)
DOI: 10.1177/00220345940730100301


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