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Journal of Dental Research
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Effect of Centrifugal Force on Growth of Mouse Osteoblastic MC3T3-E1 Cells in vitro

H. Inoue

Department of Orthodontics

O. Nakamura

Department of Orthodontics

Y. Duan

Department of Orthodontics, Qindu Stomatological College, Xian, Shaanxi, China

Y. Hiraki

Biochemistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

M. Sakuda

Department of Orthodontics

The effect of biomechanical force on growth of skeletal tissue was studied in monolayer cultures of mouse osteoblastic MC3T3-E1 cells which were centrifuged at 320 g for 15 min to 72 h in a CO2 incubator. Centrifugation of the cells for 30 min in low concentrations (0.3 or 1%) of fetal bovine serum (FBS) caused a two-fold increase of [3H]thymidine incorporation at 20 h from the start of centrifugation. However, centrifugation under 10% FBS caused no increase in [3H]thymidine incorporation into DNA. Under 0.3% FBS, [3H]thymidine incorporation increased in a manner dependent on the period of centrifugation and reached a maximum when the cells were centrifuged for 3 h. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. Moreover, conditioned medium collected from the centrifuged cultures increased [3H]thymidine incorporation by two-fold over the basal when added to a quiescent culture of MC3T3-E1 cells. These results suggest that centrifugal force stimulates growth of osteoblastic cells through autocrine secretion of some diffusible growth-promoting activity. On the other hand, centrifugation of the cells inhibited induction by FBS of alkaline phosphatase activity and calcium-uptake, two indices of the differentiated phenotype of osteoblasts.

Journal of Dental Research, Vol. 72, No. 9, 1351-1355 (1993)
DOI: 10.1177/00220345930720091601
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