This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Spielman, A.I. Articles by Schmale, H. Search for Related Content PubMed PubMed Citation Articles by Spielman, A.I. Articles by Schmale, H. Social Bookmarking                         What's this?

Protein Analysis of Human von Ebner Saliva and a Method for its Collection from the Foliate Papillae

Division of Basic Sciences, New York University College of Dentistry, 345 E. 24th Street, New York, New York 10010

S. D'Abundo

Division of Basic Sciences, New York University College of Dentistry, 345 E. 24th Street, New York, New York 10010

R.B. Field

Georgetown University Medical Center, Department of Pediatrics, and Department of Veterans Affairs Medical Center, Oral Pathology Research Lab., Washington, DC

H. Schmale

University of Hamburg, Department of Cell Biology and Clinical Neurobiology, Hamburg, Germany

The lingual serous glands of von Ebner are located close to the foliate and circumvallate papillae. Saliva secreted by these glands provides the immediate environment of the taste buds, and it has been hypothesized that it modulates taste perception. The purpose of this study was to develop a technique for collection of unstimulated and stimulated saliva from human von Ebner glands. Saliva was collected under resting conditions and after application of various gustatory stimuli (sweet, sour, salt, and bitter) by insertion of periostrips into the folds of the foliate papillae of healthy human volunteers. Stimulated saliva was also collected in glass microcapillaries or micropipettes. The flow-rates of unstimulated von Ebner saliva were 2.3 ± 0.6 (S.E.) µL/min and 4.5 ± 1.2 (S.E.) µL/min with 1% citric acid stimulation. The protein content was 2.5 ± 0.5 (S.E.) mg/mL. The SDS gel electrophoretic profile of von Ebner saliva revealed two protein bands of Mr 18,000 that were identified on Western blots as von Ebner gland (VEG) proteins. Although lingual lipase activity was detected at very low levels by enzyme assay, this protein was not detected on Western blots. This collection technique should prove useful for analysis of specific functions associated with secretion from von Ebner glands.

Journal of Dental Research, Vol. 72, No. 9, 1331-1335 (1993)
DOI: 10.1177/00220345930720091301


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R. D. Mattes Oral Detection of Short-, Medium-, and Long-Chain Free Fatty Acids in Humans Chem Senses, February 1, 2009; 34(2): 145 - 150. [Abstract] [Full Text] [PDF]

				R. D. Mattes Effects of linoleic acid on sweet, sour, salty, and bitter taste thresholds and intensity ratings of adults Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1243 - G1248. [Abstract] [Full Text] [PDF]

				J. Leinonen, S. Parkkila, K. Kaunisto, P. Koivunen, and H. Rajaniemi Secretion of Carbonic Anhydrase Isoenzyme VI (CA VI) from Human and Rat Lingual Serous von Ebner's Glands J. Histochem. Cytochem., May 1, 2001; 49(5): 657 - 662. [Abstract] [Full Text]

				R. Matsuo Role of Saliva in the Maintenance of Taste Sensitivity Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(2): 216 - 229. [Abstract] [Full Text] [PDF]

				E. T. Rolls, H. D. Critchley, A. S. Browning, I. Hernadi, and L. Lenard Responses to the Sensory Properties of Fat of Neurons in the Primate Orbitofrontal Cortex J. Neurosci., February 15, 1999; 19(4): 1532 - 1540. [Abstract] [Full Text] [PDF]

				A.I. Spielman Chemosensory Function and Dysfunction Critical Reviews in Oral Biology & Medicine, January 1, 1998; 9(3): 267 - 291. [Abstract] [Full Text] [PDF]