This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hakkinen, L. Articles by Larjava, H. Search for Related Content PubMed PubMed Citation Articles by Hakkinen, L. Articles by Larjava, H. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

Characterization of Fibroblast Clones from Periodontal Granulation Tissue in vitro

Departments of Oral Biology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland

H. Larjava

Department of Periodontology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland

Connective tissues are known to be composed of heterogeneous fibroblast subpopulations. The significance of this heterogeneity in different physiological and pathological conditions is poorly understood. Granulation tissue is formed in connective tissue during wound healing, chronic inflammation, and certain pathological conditions. In this study, heterogeneity of fibroblasts from granulation tissue was investigated by cell-cloning techniques. Granulation-tissue fibroblasts (GTFs) from both chronically inflamed periodontal lesions and healing wound granulation tissue behaved similarly. GTFs showed a more pronounced decrease in proliferative capacity with increasing cumulative population doubling levels (CPDLs) and 30-40% lower cloning efficiency compared with normal gingival fibroblasts (HGFs). Morphologically, cells in GTF cultures were mainly large, whereas HGFs were mainly small in size. Both cell-line types showed heterogeneity in cell morphology. Clones composed of large stellate-like cells predominated in GTF cultures, whereas clones composed of small spindle-shaped or epithelioid cells predominated in HGF cultures. In both cell-line types, the proportion of clones composed of large cells increased with increasing CPDL. These findings show that the properties of the fibroblasts changed during their in vitro life spans. The finding that normal connective tissue and granulation tissues contain morphologically distinct fibroblast clones in different proportions suggests that local factors could stimulate local fibroblasts to differentiate into GTFs. Alternatively, local factors could select some fibroblast subpopulations to overgrow the others to form granulation tissue.

Journal of Dental Research, Vol. 71, No. 12, 1901-1907 (1992)
DOI: 10.1177/00220345920710120901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M. Das, E. C. Dempsey, J. T. Reeves, and K. R. Stenmark Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L976 - L986. [Abstract] [Full Text] [PDF]

				L. Kuru, M.H. Parkar, G.S. Griffiths, H.N. Newman, and I. Olsen Flow Cytometry Analysis of Gingival and Periodontal Ligament Cells Journal of Dental Research, April 1, 1998; 77(4): 555 - 564. [Abstract] [PDF]

				P.C. Lekic, N. Pender, and C.A.G. McCulloch Is Fibroblast Heterogeneity Relevant To the Health, Diseases, and Treatments of Periodontal Tissues? Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(3): 253 - 268. [Abstract] [Full Text] [PDF]

				L. Hakkinen, J. Westermarck, V.-M. Kahari, and H. Larjava Human Granulation-tissue Fibroblasts Show Enhanced Proteoglycan Gene Expression and Altered Response to TGF-{beta}1 Journal of Dental Research, October 1, 1996; 75(10): 1767 - 1778. [Abstract] [PDF]

				C. Giannopoulou and G. Cimasoni Functional Characteristics of Gingival and Periodontal Ligament Fibroblasts Journal of Dental Research, March 1, 1996; 75(3): 895 - 902. [Abstract] [PDF]

				M. Makela, T. Salo, V.-J. Uitto, and H. Larjava Matrix Metalloproteinases (MMP-2 and MMP-9) of the Oral Cavity: Cellular Origin and Relationship to Periodontal Status Journal of Dental Research, August 1, 1994; 73(8): 1397 - 1406. [Abstract] [PDF]

				C. Irwin, M Picardo, I Ellis, P Sloan, A Grey, M McGurk, and S. Schor Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing J. Cell Sci., January 5, 1994; 107(5): 1333 - 1346. [Abstract] [PDF]