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An in vitro Model for Assessment of Fluoride Uptake from Glass-ionomer Cements by Dentin and its Effect on Acid ResistanceDepartments of Pediatric Dentistry and Oral Diagnosis, University of Alabama School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294
Departments of Pediatric Dentistry and Oral Diagnosis, University of Alabama School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294 This investigation presents an experimental model for studying interactions of glass-ionomer cements (GICs) with bovine dentin slabs. Fluoride incorporation was studied with five serial abrasion biopsies, each being approximately 10 µm thick. The time of interaction was a very important parameter, indicating continuous fluoride release from the GIC and diffusion into dentin over a 30-day period. Expressed in mass per volume (mg F/cm3), the fluoride incorporation reached 12.0 mg at the first and 2.5 mg at the fifth layer, several times greater than the baseline of 0.27 mg/cm3 in bovine dentin. A subsequent test of acid resistance in a lactic acid buffer (pH 4.0), followed by microradiography of lesions, showed a characteristic 40-µm-wide acid-resistant zone on surfaces exposed to the GICs. In contrast, the untreated control surfaces had lesions demineralized evenly from the surface to the intact tissue, without the higher-density zone at the surface of the lesion. The model seems promising for screening fluoride incorporation into dentin from fluoride-releasing dental materials.
Journal of Dental Research, Vol. 71, No. 1,
7-12 (1992) This article has been cited by other articles:
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