This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Liu, Y. Articles by Grafström, R.C. Search for Related Content PubMed PubMed Citation Articles by Liu, Y. Articles by Grafström, R.C. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CYSTEINE MERCURY COMPOUNDS MERCURY, ELEMENTAL Social Bookmarking                         What's this?

Development of Low- and High-serum Culture Conditions for Use of Human Oral Fibroblasts in Toxicity Testing of Dental Materials

Department of Toxicology, Karolinska Institutet, Box 60 400, S-104 01, Stockholm, Sweden

K. Arvidson

Department of Prosthodontics, Karolinska Institutet, Box 4064, S-141 04, Huddinge, Sweden

L. Atzori

Department of Toxicology, Karolinska Institutet, Box 60 400, S-104 01, Stockholm, Sweden

K. Sundqvist

Department of Toxicology, Karolinska Institutet, Box 60 400, S-104 01, Stockholm, Sweden

B. Silva

Department of Prosthodontics, Karolinska Institutet, Box 4064, S-141 04, Huddinge, Sweden

I. Cotgreave

Department of Toxicology, Karolinska Institutet, Box 60 400, S-104 01, Stockholm, Sweden

R.C. Grafström

Department of Toxicology, Karolinska Institutet, Box 60 400, S-104 01, Stockholm, Sweden

With the aim of establishing conditions applicable to the testing of dental materials in human target cells, fibroblastic cell lines have been derived and grown from explants of human oral mucosa. Both a high-serum medium (termed "HSM") (CMRL 1066 supplemented with 10% fetal bovine serum) and a low-serum medium (termed "LSM") (a 1:1 mixture of M 199:MCDB 153 supplemented with 1.25% serum) supported radial outgrowths of cells from oral explants, as well as the subsequent transfer and growth of the cells in mass culture and at clonal density. Cells were typically fibroblastic in that they expressed vimentin uniformly, but did not express immunocytochemical markers of epithelial or endothelial cells. Cells derived in either LSM or HSM showed significantly higher colony-forming efficiency and clonal growth rate when transferred in LSM, as compared with HSM. Because cell migration occurred to a lesser extent in LSM, microscopic scoring of colony formation was also markedly facilitated. In both LSM and HSM, cellular low-molecular-weight thiols constituted about 30% of the total amount of sulfhydryls. Glutathione was present in about six- to seven-fold-higher amounts than cysteine-glutathione primarily in its reduced form and cysteine primarily in its oxidized form. A corrosion product of dental amalgam, i.e., Hg2+, decreased cell survival measured as colony-forming efficiency in a dose-dependent manner following either an acute (one h) exposure or continuous exposure (seven days). These studies demonstrated that human oral fibroblasts could be cultured at about one-tenth of the serum content that is commonly used. It is concluded that human oral fibroblasts grown at low-serum conditions provide a highly sensitive and easily applicable in vitro model for toxicity studies of dental materials.

Journal of Dental Research, Vol. 70, No. 7, 1068-1073 (1991)
DOI: 10.1177/00220345910700071101


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M.M. Barczyk, L.-H. B. Olsen, P. da Franca, B.G. Loos, K. Mustafa, D. Gullberg, and A.I. Bolstad A Role for {alpha}11{beta}1 Integrin in the Human Periodontal Ligament Journal of Dental Research, July 1, 2009; 88(7): 621 - 626. [Abstract] [Full Text] [PDF]

				M. Vondracek, Z. Xi, P. Larsson, V. Baker, K. Mace, A. Pfeifer, H. Tjalve, M.T. Donato, M.J. Gomez-Lechon, and R. C. Grafstrom Cytochrome P450 expression and related metabolism in human buccal mucosa Carcinogenesis, March 1, 2001; 22(3): 481 - 488. [Abstract] [Full Text] [PDF]

				J. J. Hedberg, J.-O. Hoog, J. A. Nilsson, Z. Xi, A. Elfwing, and R. C. Grafstrom Expression of Alcohol Dehydrogenase 3 in Tissue and Cultured Cells from Human Oral Mucosa Am. J. Pathol., November 1, 2000; 157(5): 1745 - 1755. [Abstract] [Full Text] [PDF]

				J.A. Nilsson, X. Zheng, K. Sundqvist, Y. Liu, L. Atzori, A. Elfwing, K. Arvidson, and R.C. Grafstrom Toxicity of Formaldehyde to Human Oral Fibroblasts and Epithelial Cells: Influences of Culture Conditions and Role of Thiol Status Journal of Dental Research, November 1, 1998; 77(11): 1896 - 1903. [Abstract] [PDF]