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Effects of Calcium-phosphate-based Materials on Proliferation and Alkaline Phosphatase Activity of Newborn Rat Periosteal Cells in vitro

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Tarquilio

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy, Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

M. Grano

Centro di Citomorfologia Normale e Patologica, University of Chieti, Italy

S. Colucci

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Laforgia

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

F. Mangini

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Zambonin Zallone

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

The effects of dental materials, intended for bone substitution, on cell growth and alkaline phosphatase activity of newborn rat periosteal cells have been studied in vitro. Confluent periosteal cells were exposed to three apatite-based materials (400 µg/mL) with different physico-chemical properties. The materials were a β-tricalcium phosphate with a microporous granular structure obtained by sinterization (Synthograft, Johnson & Johnson, East Windsor, NY), a 40-60-mesh microporous durapatite ceramic (Periograf, Sterling Drug, Inc., Rensselaer, NY), and a 1-2-mm-diameter hydroxyapatite ceramic (Osprovit, Feldmuhle Aktiengeselschaft, Plochingen, Germany) with macropores larger than 100 µm. Cell proliferation and alkaline phosphatase activity were assessed by incorporation of 3H-thymidine into trichloroacetic-acid-precipitable material and by a fluorimetric method, respectively. Cell viability and compatibility with the materials were determined by morphology in phase-contrast microscopy. Periosteal cells showed increased proliferation following exposure to Synthograft, but were unaffected by Osprovit, whereas Periograf caused significantly reduced cell growth. Alkaline phosphatase activity was unaffected by Osprovit, but was decreased by both Synthograft and Periograf. The results indicated a differential response of periosteal cells to bone-substituting materials with heterogeneous physico-chemical characteristics.

Journal of Dental Research, Vol. 70, No. 6, 997-1001 (1991)
DOI: 10.1177/00220345910700061801


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