This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Teti, A. Articles by Zambonin Zallone, A. Search for Related Content PubMed PubMed Citation Articles by Teti, A. Articles by Zambonin Zallone, A. Social Bookmarking                         What's this?

Effects of Calcium-phosphate-based Materials on Proliferation and Alkaline Phosphatase Activity of Newborn Rat Periosteal Cells in vitro

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Tarquilio

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy, Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

M. Grano

Centro di Citomorfologia Normale e Patologica, University of Chieti, Italy

S. Colucci

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Laforgia

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

F. Mangini

Clinica Odontostomatologica, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

A. Zambonin Zallone

Institute of Human Anatomy, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

The effects of dental materials, intended for bone substitution, on cell growth and alkaline phosphatase activity of newborn rat periosteal cells have been studied in vitro. Confluent periosteal cells were exposed to three apatite-based materials (400 µg/mL) with different physico-chemical properties. The materials were a β-tricalcium phosphate with a microporous granular structure obtained by sinterization (Synthograft, Johnson & Johnson, East Windsor, NY), a 40-60-mesh microporous durapatite ceramic (Periograf, Sterling Drug, Inc., Rensselaer, NY), and a 1-2-mm-diameter hydroxyapatite ceramic (Osprovit, Feldmuhle Aktiengeselschaft, Plochingen, Germany) with macropores larger than 100 µm. Cell proliferation and alkaline phosphatase activity were assessed by incorporation of 3H-thymidine into trichloroacetic-acid-precipitable material and by a fluorimetric method, respectively. Cell viability and compatibility with the materials were determined by morphology in phase-contrast microscopy. Periosteal cells showed increased proliferation following exposure to Synthograft, but were unaffected by Osprovit, whereas Periograf caused significantly reduced cell growth. Alkaline phosphatase activity was unaffected by Osprovit, but was decreased by both Synthograft and Periograf. The results indicated a differential response of periosteal cells to bone-substituting materials with heterogeneous physico-chemical characteristics.

Journal of Dental Research, Vol. 70, No. 6, 997-1001 (1991)
DOI: 10.1177/00220345910700061801


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?