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Association of Gingival Crevicular Fluid Aspartate Aminotransferase Levels with Histopathology during Ligature-induced Periodontitis in the Beagle Dog

Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Departments of Oral Medicine and Diagnostic Sciences, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Department of Dermatology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612

M.E.A.F. Alves

Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Department of Periodontics, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612

J.M. Crawford

Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Department of Periodontics, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612

T. McSwiggin

Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612

D.A. Chambers

Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Department of Dermatology, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612, Department of Biochemistry, University of Illinois at Chicago, 801 S. Paulina Street, Chicago, Illinois 60612

Previous investigations have shown a clear association between the presence of the enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) and clinical evidence of periodontal disease in humans, as well as in the beagle dog model. This paper describes a 26-week study that uses the beagle dog model of ligature-induced periodontitis in which GCF-AST (corrected for collection time) was correlated with microscopic evidence of tissue destruction in the periodontium at the sites of fluid collection. GCF and clinical data were collected at baseline, at optimal gingival health, during gingivitis, and after ligation. A cross-mouth design was implemented so that six premolar teeth in each dog were ligated for periods up to five weeks. Formalin-fixed tissues from the sites of GCF collection were prepared for light microscopy and evaluated for the presence of epithelial ulceration, bone resorption, and inflammatory cell infiltration. The relationship between GCF-AST levels and microscopic findings was analyzed by calculation of sensitivity and specificity and by plots of Receiver Operating Characteristics. These data revealed a correlation between elevated enzyme concentration and microscopic evidence of disease activity. Taken together with human studies, these results provide support for the use of AST as a marker of periodontal disease progression.

Journal of Dental Research, Vol. 70, No. 6, 984-987 (1991)
DOI: 10.1177/00220345910700061501


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