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Journal of Dental Research
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Quantitative Immunohistochemical Analysis of Keratins and Desmoplakins in Human Gingiva and Peri-implant Mucosa

R.P. Carmichael

Departments of Prosthodontics, University of Toronto, 124 Edward Street, Toronto, Ontario, Canada M5G 1G6

C.A.G. McCulloch

Periodontics, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, Ontario, Canada M5G 1G6

G.A. Zarb

Departments of Prosthodontics, University of Toronto, 124 Edward Street, Toronto, Ontario, Canada M5G 1G6

Quantitative immunohistochemistry was used to compare the distributions of keratins and desmoplakins in human gingiva and peri-implant mucosa (three specimens each). In gingiva, keratin 1 (a marker of cornification) and desmoplakins I & II (markers of desmosomes) stained most heavily in granular strata followed by corneal strata; keratin 13, a marker of non-cornifying stratified squamous cells, stained most heavily in suprabasal strata of oral sulcular epithelium. Keratin 19, a marker for junctional epithelium, stained the basal stratum of oral sulcular epithelium most heavily. In peri-implant mucosa, the patterns of staining were similar, except that staining for desmoplakins I & II was generally significantly reduced compared with gingiva, and junctional epithelium co-expressed keratins 13 and 19. Peri-implant junctional epithelial cells attached to titanium implant abutments were removed by trypsin/EDTA digestion, and also exhibited co-expression of keratins 13 and 19. Inflammatory cell infiltration was associated with reduction of keratin 1 staining in gingiva. The data indicate that the epithelia of gingiva and peri-implant mucosa are not composed of identical cell populations.

Journal of Dental Research, Vol. 70, No. 5, 899-905 (1991)
DOI: 10.1177/00220345910700050701
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