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A Human Periodontal Ligament Fibroblast Clone Releases a Bone Resorption Inhibition Factor in vitro

Departments of BioStructure and Function, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

L. Norton

Department of Orthodontics, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

S. Sousa

Department of Medicine, The Veterans Administration Medical Center, Newington, Connecticut 06111

J.A. Lorenzo

Department of Medicine, The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030, Department of Medicine, The Veterans Administration Medical Center, Newington, Connecticut 06111

F. Bronner

Departments of BioStructure and Function, , The University of Connecticut Health Center, Farmington Avenue, Farmington, Connecticut 06030

The conditioned media (CM) obtained from three lines of cloned human periodontal ligament (PDL) cells were analyzed to determine whether they altered the parathyroid hormone (PTH)-stimulated resorption rates (45Ca release) in 48-hour cultures of ⁴⁵Ca-labeled rat long bones. One PDL cell line, PDL-5, produced a heat-resistant factor in its CM that inhibited the PTH-stimulated resorption by 43.8 ± 9.7 (SE) percent (p < 0.02), whereas the CM from the other cell lines were without statistically significant effect. The CM from the PDL-5 line did not diminish organ culture viability, as determined by 3H-thymidine incorporation, and did not enhance or diminish the resorption-inhibiting activity of calcitonin added to the PTH-stimulated cultures. The addition of CM from PDL-5 did not alter the bone-resorbing effect of interleukin-1 (IL-1). These results indicate that CM from PDL-5 inhibits only the PTHinduced and not the IL-1-mediated resorption processes, whose mechanisms are therefore likely to differ.

Journal of Dental Research, Vol. 70, No. 2, 99-101 (1991)
DOI: 10.1177/00220345910700021901


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