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Cytotoxic Effects of Resin Components on Cultured Mammalian Fibroblasts

Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078

S.E. Strawn

Departments of Oral Medicine, Pathology and Surgery, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078

J.C. Watahai

Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078

R.G. Craig

Biological and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078

The objectives of this study were to determine the cytotoxic concentrations of 11 components of resin composites on monolayers of cultured Balb/c 3T3 fibroblasts, to study the inhibitory effects of these components on DNA synthesis, total protein content, and protein synthesis, and to determine whether effects were reversible when the components were withdrawn from the medium. These data were reported as concentrations which inhibited 10% (ID₁₀) and 50% (ID₅₀) of a particular metabolic process as well as the range of concentrations over which cell metabolism was irreversibly inhibited. For any individual component, the ID50 values for all three metabolic parameters were of the same magnitude. The same was true for the ranges of irreversibility. Ethoxylated Bis-phenol A dimethacrylate (E-BPA) was the most toxic molecule of the group (ID₅₀ being between 1 and 10 µmol/L). The ID₅₀ concentrations for three of the components, including Bis-GMA, UDMA, TEGDMA, and Bis-phenol A, ranged between 10 and 100 µmol/L, while the ID ₅₀ values of three components (N,N dihydroxyethyl-p-toluidine, camphoroquinone, and N,N dimethylaminoethyl methacrylate) were above 100 µmol/L. The concentrations to which the cells and tissues are exposed in uiuo are not known. This study should help to identify the concentrations of organic composite components which pose clinical cytotoxic hazards.

Journal of Dental Research, Vol. 70, No. 11, 1450-1455 (1991)
DOI: 10.1177/00220345910700111201


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