This Article Free Full Text (Free PDF) Free References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Okiji, T. Articles by Murota, S. Search for Related Content PubMed PubMed Citation Articles by Okiji, T. Articles by Murota, S. Social Bookmarking                         What's this?

The Role of Leukotriene B4 in Neutrophil Infiltration in Experimentally-induced Inflammation of Rat Tooth Pulp

Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan, Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

I. Morita

Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

I. Sunada

Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

S. Murota

Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-Ku, Tokyo 113, Japan

Inflammation was induced in rat dental pulp by applying bacterial lipopolysaccharide (LPS). Extirpated tissue samples from inflamed pulps were incubated in vitro in a Krebs buffer containing Ca2+ ionophore A23187, and leukotriene (LT) B₄ released into the medium was determined by radio-immunoassay. Production of LTB4 could be detected three to 24 h after the application of LPS and showed a maximum at 12 h. Histologically, marked infiltration of neutrophils, but not other leukocytes, was characteristically observed in the LPS-applied pulps, and the temporal change in neutrophil infiltration was almost parallel, but somewhat more delayed than LTB4 production. When BW755C, a dual inhibitor of cyclo-oxygenase and lipoxygenase, was given to the animals before the application of LPS, both the production of LTB4 and the number of infiltrated neutrophils were significantly decreased, whereas administration of indomethacin had no effect. These results suggest that LTB4 may be involved in neutrophil infiltration in pulpal inflammation. It was also suggested that a major early source of LTB4 in experimental pulpitis was leukocytes, primary neutrophils, because the synthesis of LTB4 in the inflamed pulp was diminished by depletion of circulating leukocytes with cyclophosphamide prior to the application of LPS.

Journal of Dental Research, Vol. 70, No. 1, 34-37 (1991)
DOI: 10.1177/00220345910700010501


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				P.N.R. Nair PATHOGENESIS OF APICAL PERIODONTITIS AND THE CAUSES OF ENDODONTIC FAILURES Critical Reviews in Oral Biology & Medicine, November 1, 2004; 15(6): 348 - 381. [Abstract] [Full Text] [PDF]