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Stimulation of Proteoglycan and DNA Syntheses in Chondrocytes by Centrifugation

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

K. Hiasa

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

Y. Samma

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

O. Nakamura

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

M. Sakuda

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

M. Iwamoto

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

F. Suzuki

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

Y. Kato

Department of Orthodontics and Biochemistry, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, Suita, Osaka 565, Japan

So that the effects of biomechanical forces on the proliferation of chondrocytes and their proteoglycan synthesis could be studied, growth-plate and articular chondrocytes were maintained separately as packed masses in centrifuge tubes in the presence of 10% serum. In these conditions, the cells became re-organized into cartilaginous tissue in seven days. After ten days, they were centrifuged at gravities (g) of 1.3-27 for 24 h in a CO2 incubator. Control cells were maintained in the CO2 incubator without centrifugation. Centrifugation of growth-plate chondrocytes at 3 g resulted in a two-fold increase in incorporation of [³⁵S]sulfate into proteoglycans, but had little effect on their [³H]thymidine incorporation into DNA. On the other hand, centrifugation of articular chondrocytes at 3 g for 24 h caused 1.5-fold increases in both [³⁵S]sulfate incorporation into proteoglycans and [3H]thymidine incorporation into DNA. These results suggest that biomechanical forces have different effects on the growth and differentiation of articular and growth-plate chondrocytes.

Journal of Dental Research, Vol. 69, No. 9, 1560-1563 (1990)
DOI: 10.1177/00220345900690090401


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