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Journal of Dental Research
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Optical Quantification of Caries-like Lesions in vitro by Use of a Fluorescent Dye

J.W. Van de Rijke

Laboratory for Materia Technica, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

J.J. Ten Bosch

Laboratory for Materia Technica, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands

An experimental method was developed for measurement of the fluorescence intensity of a dye that was introduced into caries-like lesions in vitro. A distinct pattern of change of fluorescence intensity with time appeared, displaying a plateau value and a peak value for each measurement. Both plateau and peak values showed a linear correlation with calcium loss, as measured with longitudinal microradiography. The correlation coefficients were r = 0.87 for plateau values and r = 0.89 for peak values. The difference in scattering by dry and wet caries lesions was also measured with the same equipment, which showed a linear correlation with calcium loss of r = -0.53.

Journal of Dental Research, Vol. 69, No. 5, 1184-1187 (1990)
DOI: 10.1177/00220345900690051201
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J.J. Ten Bosch and B. Angmar-Mansson
Invited Review: A Review of Quantitative Methods for Studies of Mineral Content of Intra-oral Incipient Caries Lesions
Journal of Dental Research, January 1, 1991; 70(1): 2 - 14.
[Abstract] [PDF]