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The Effect of Sucrose Application and Implantation of Mutans Streptococci on the Microbial Composition of Three-week Experimental Plaque—An in situ Study

Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom

T.W. MacFarlane

Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom

T.C. Aitchison

Department of Statistics, University of Glasgow, University Avenue, Glasgow G12 8QQ, Scotland, United Kingdom

K.W. Stephen

Department of Oral Medicine and Pathology, University of Glasgow Dental Hospital and School, 378 Sauchiehall Street, Glasgow G2 3JZ, Scotland, United Kingdom

This study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. Lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. These were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans streptococci onto the enamel test sites and sucrose applications, as described above. With the exception of slightly higher proportions of Gram-negative bacilli associated with slab plaque following sucrose application, no significant differences in percentage or absolute counts of organisms were found between normal and sucrose plaques. The inoculation of mutans streptococci, combined with extra-oral sucrose applications, was associated with significantly higher percentages and absolute mean counts of both mutans streptococci and lactobacilli, and lower proportions of S. sanguis and S. oralis. Although the isolation frequency of mutans streptococci increased in all subjects and the overall mean proportion rose following inoculation, considerable inter-subject variation was seen in mean percentage counts of these organisms isolated from the three-week plaque samples.

Journal of Dental Research, Vol. 69, No. 11, 1706-1711 (1990)
DOI: 10.1177/00220345900690110101


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