This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Turner, D.F. Articles by Sattelberg, C. Search for Related Content PubMed PubMed Citation Articles by Turner, D.F. Articles by Sattelberg, C. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat

Department of Oral Biology, The University of Michigan Dental School, Ann Arbor, Michigan 48109-1078

C.F. Marfurt

Department of Anatomy, Indiana University School of Medicine, Northwest Center for Medical Education, Gary, Indiana 46408

C. Sattelberg

Department of Oral Biology, The University of Michigan Dental School, Ann Arbor, Michigan 48109-1078

Vascular injection of the macromolecular tracer, horseradish peroxidase (HRP), was used to study the permeability of the odontoblast cell layer in developing and mature rat molar teeth, and to investigate the effect of cavity preparations on the permeability of this epithelioid cell layer in adult animals. HRP injected into the vascular system of normal animals 28 days of age and older was localized histochemically (from 5 to 90 min after injection) throughout the extracellular spaces of the maxillary dental pulps; however, the tracer did not penetrate beyond the tight junctions at the apical region of the odontoblast cell layer, and was absent from the predentin and dentin. In contrast, HRP injected into very young neonatal animals (e.g., day 3) resulted in free passage of HRP between odontoblasts and into the overlying predentin and dentin. When Class V cavities had been prepared in adult maxillary molars after HRP was injected into the blood stream, HRP reaction product penetrated the predentin and dentin immediately beneath the cavity preparation; however, adjacent, un-traumatized areas of predentin and dentin in the operated teeth were devoid of reaction product. These results provide evidence that: (1) a physiological barrier develops between the distal segments of odontoblast cell bodies in normal rat molar teeth between days 15 and 28 of postnatal life, and this barrier prevents the passage of macromolecules from the pulp into the predentin and dentin; and (2) this barrier is perturbed following routine restorative procedures in adult animals.

Journal of Dental Research, Vol. 68, No. 8, 1262-1268 (1989)
DOI: 10.1177/00220345890680081001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Bouillaguet BIOLOGICAL RISKS OF RESIN-BASED MATERIALS TO THE DENTIN-PULP COMPLEX Critical Reviews in Oral Biology & Medicine, January 1, 2004; 15(1): 47 - 60. [Abstract] [Full Text] [PDF]

				G. Bergenholtz Evidence for Bacterial Causation of Adverse Pulpal Responses in Resin-Based Dental Restorations Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(4): 467 - 480. [Abstract] [Full Text] [PDF]

				D.H. Pashley Dynamics of the Pulpo-Dentin Complex Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(2): 104 - 133. [Abstract] [Full Text] [PDF]