This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Field, R.B. Articles by Hand, A.R. Search for Related Content PubMed PubMed Citation Articles by Field, R.B. Articles by Hand, A.R. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

Purification of Lingual Amylase from Serous Glands of Rat Tongue and Characterization of Rat Lingual Amylase and Lingual Lipase

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892

A.I. Spielman

Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8

A.R. Hand

National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892

Lingual amylase and lingual lipase, two digestive enzymes that are secreted from lingual serous glands (von Ebner's), were simultaneously purified from rat lingual serous glands with hydrophobic chromatography used as the final step. This method, previously developed for the purification of lingual lipase, includes homogenization of rat lingual serous glands, 100,000 g centrifugation, ammonium sulfate precipitation of proteins, and extraction of lipids with acetone at -20°C, followed by hydrophobic chromatography on ethyl agarose or Agethane. Amylase was eluted after the elution of proteins that did not interact with the hydrophobic gel at pH 6.3. Lingual lipase was eluted with a solution containing micelles of taurodeoxycholate, monoolein, and oleic acid. Analysis of each of the purified enzymes by SDS polyacrylamide gel electrophoresis revealed one band at M, = 59,000 for amylase and one band at M, = 51, 000 for lingual lipase. Isoelectric focusing of amylase indicated a strong band at pl = 5.0 and two very faint bands at pl = 4.9 and 4.8, possibly isozymes or deamidated protein. Amino acid and hexosamine analyses were performed on the enzymes after electroelution from SDS-polyacrylamide gels. Both lingual lipase and lingual amylase had a high content of dicarboxylic (free and amide) amino acids. For lingual lipase and lingual amylase, the % molar ratios of aspartic acid/asparagine were 15.35 and 15.10, and the % molar ratios of glutamic acid/glutamine were 7.07 and 7.20, respectively. Lingual amylase was very similar to rat parotid, pancreatic, and mouse salivary amylases, except that it contained more proline (11.03% molar ratio). Although no hexosamines were detected in lingual amylase, lingual lipase contained 14 moles of hexosamines/mole, 10 moles of GlcNAc, and 4 moles of GaINAc, indicating that the enzyme undergoes both N-linked and 0-linked glycosylation.

Journal of Dental Research, Vol. 68, No. 2, 139-145 (1989)
DOI: 10.1177/00220345890680020801


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Suwabe, H. Fukami, and R. M. Bradley Synaptic Responses of Neurons Controlling the Parotid and von Ebner Salivary Glands in Rats to Stimulation of the Solitary Nucleus and Tract J Neurophysiol, March 1, 2008; 99(3): 1267 - 1273. [Abstract] [Full Text] [PDF]

				A.I. Spielman, S. D'Abundo, R.B. Field, and H. Schmale Protein Analysis of Human von Ebner Saliva and a Method for its Collection from the Foliate Papillae Journal of Dental Research, September 1, 1993; 72(9): 1331 - 1335. [Abstract] [PDF]

				A.I. Spielman Interaction of Saliva and Taste Journal of Dental Research, March 1, 1990; 69(3): 838 - 843. [Abstract] [PDF]