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Characterization of One Phenotype of Human Periodontal Granulation-tissue Fibroblasts

Department of Periodontology, University of Turku, SF-20520 Turku

J. Heino

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

V.-M. Kähäri

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

T. Krusius

Department of Medical Chemistry, University of Helsinki, SF-00170 Helsinki, Finland

E. Vuorio

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

Human granulation-tissue fibroblasts were cultured from oral chronic inflammatory lesions and compared with fibroblasts of healthy gingival connective tissue with respect to cell-surface sialoglycoproteins, and the synthesis of extracellular matrix components. Granulation-tissue fibroblasts exhibited a slower growth rate and larger size than their controls. Their cell-surface sialoglycoproteins resembled those of the control cells, except that the relative amount of glycoproteins in the 140-kd region was lower. The ratio of mRNAs for prol(I) and prol(III) collagen chains was decreased in granulation-tissue fibroblasts, although electrophoretic fractionation of the proteins did not reveal consistent differences in type I/type III collagen ratio. Granulation-tissue fibroblasts secreted into the culture medium a dermatan sulfate proteoglycan with a lower molecular weight. After digestion with chondroitinase ABC, the molecular weight of the core protein appeared to be identical with that of the control fibroblasts, suggesting a difference in the glycosylation of the core protein. These results support the theory that granulation-tissue fibroblasts represent a distinct phenotype of fibroblastic cells.

Journal of Dental Research, Vol. 68, No. 1, 20-25 (1989)
DOI: 10.1177/00220345890680010301


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