This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Larjava, H. Articles by Vuorio, E. Search for Related Content PubMed PubMed Citation Articles by Larjava, H. Articles by Vuorio, E. Social Bookmarking                         What's this?

Characterization of One Phenotype of Human Periodontal Granulation-tissue Fibroblasts

Department of Periodontology, University of Turku, SF-20520 Turku

J. Heino

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

V.-M. Kähäri

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

T. Krusius

Department of Medical Chemistry, University of Helsinki, SF-00170 Helsinki, Finland

E. Vuorio

Department of Medical Biochemistry, University of Turku, SF-20520 Turku

Human granulation-tissue fibroblasts were cultured from oral chronic inflammatory lesions and compared with fibroblasts of healthy gingival connective tissue with respect to cell-surface sialoglycoproteins, and the synthesis of extracellular matrix components. Granulation-tissue fibroblasts exhibited a slower growth rate and larger size than their controls. Their cell-surface sialoglycoproteins resembled those of the control cells, except that the relative amount of glycoproteins in the 140-kd region was lower. The ratio of mRNAs for prol(I) and prol(III) collagen chains was decreased in granulation-tissue fibroblasts, although electrophoretic fractionation of the proteins did not reveal consistent differences in type I/type III collagen ratio. Granulation-tissue fibroblasts secreted into the culture medium a dermatan sulfate proteoglycan with a lower molecular weight. After digestion with chondroitinase ABC, the molecular weight of the core protein appeared to be identical with that of the control fibroblasts, suggesting a difference in the glycosylation of the core protein. These results support the theory that granulation-tissue fibroblasts represent a distinct phenotype of fibroblastic cells.

Journal of Dental Research, Vol. 68, No. 1, 20-25 (1989)
DOI: 10.1177/00220345890680010301


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				L. Hakkinen, J. Westermarck, V.-M. Kahari, and H. Larjava Human Granulation-tissue Fibroblasts Show Enhanced Proteoglycan Gene Expression and Altered Response to TGF-{beta}1 Journal of Dental Research, October 1, 1996; 75(10): 1767 - 1778. [Abstract] [PDF]

				C. Irwin, M Picardo, I Ellis, P Sloan, A Grey, M McGurk, and S. Schor Inter- and intra-site heterogeneity in the expression of fetal-like phenotypic characteristics by gingival fibroblasts: potential significance for wound healing J. Cell Sci., January 5, 1994; 107(5): 1333 - 1346. [Abstract] [PDF]

				L. Hakkinen and H. Larjava Characterization of Fibroblast Clones from Periodontal Granulation Tissue in vitro Journal of Dental Research, December 1, 1992; 71(12): 1901 - 1907. [Abstract] [PDF]

				F. Rahemtulla Proteoglycans of Oral Tissues Critical Reviews in Oral Biology & Medicine, January 1, 1992; 3(1): 135 - 162. [Full Text] [PDF]