This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hashimoto, S. Articles by Nakamura, Y. Search for Related Content PubMed PubMed Citation Articles by Hashimoto, S. Articles by Nakamura, Y. Social Bookmarking                         What's this?

In vivo and in vitro Effects of Zinc Oxide-Eugenol (ZOE) on Biosynthesis of Cyclo-oxygenase Products in Rat Dental Pulp

Institute of Radioisotopes, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

K. Uchiyama

Department of Endodontics, School of Dentistry at Tokyo, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

M. Maeda

Department of Endodontics, School of Dentistry at Tokyo, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

K. Ishitsuka

Department of Endodontics, School of Dentistry at Tokyo, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

K. Furumoto

Institute of Radioisotopes, , The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

Y. Nakamura

Department of Endodontics, School of Dentistry at Tokyo, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102, Japan

To determine the in vivo effects of a zinc oxide-eugenol mixture (ZOE) on the cyclo-oxygenase system in dental pulp, we used radioimmunoassay to measure the levels of prostaglandin E2 (PGE2), 13,14-dihydro-15-keto-PG (DHK-PG), thromboxane B₂ (TXB2), and 6-keto-PGF₁ in the dental pulp of rats. When the dental pulp was irritated by a hole made in the dentin of the mandibular incisors without use of any coolants, the levels of these cyclo-oxygenase products in the pulp were increased to, respectively, 2.8, 1.7, 10.0, and 2.6 times those in the normal pulp at six hr after treatment. In contrast, these increases in cyclo-oxygenase products disappeared immediately when the artificial cavity in the dentin was filled with ZOE (P/L; 1 g/0.25 mL), but were not altered when the cavity was filled with zinc oxide-water (ZOW, 1 g/1.5 mL). Most of the eugenol portion of ZOE was released into the pulp within two hr after the cavity was filled with ZOE. The maximal eugenol content was 35 pmol per mg of pulp. Furthermore, when the cavity was filled either with ZOE or by the addition of 10 µmol/L eugenol to the pulp homogenate, biosynthesis of ¹⁴C-6-keto-PGF₁, PGF₂, and PGE ₂ from ¹⁴C-arachidonic acid in the homogenate was inhibited. These results suggest that eugenol released from ZOE in the cavity prepared in the dentin inhibited the biosynthesis of cyclo-oxygenase products during pulp irritation.

Journal of Dental Research, Vol. 67, No. 8, 1092-1096 (1988)
DOI: 10.1177/00220345880670080601


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Okiji, I. Morita, I. Sunada, and S. Murota The Role of Leukotriene B4 in Neutrophil Infiltration in Experimentally-induced Inflammation of Rat Tooth Pulp Journal of Dental Research, January 1, 1991; 70(1): 34 - 37. [Abstract] [PDF]