|
Sign In to gain access to subscriptions and/or personal tools.
|
Attachment of Bacteroides gingivalis to Collagenous Substrata
Y. Naito
Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115
R.J. Gibbons
Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115
The ability of Bacteroides gingivalis 381 to attach to hydroxyapatite (HA) beads, treated with either human type I or type IV collagen, or to particles of bovine bone collagen was studied. All preparations were blocked with human albumin prior to being incubated with 3H-thymidine-labeled B. gingivalis 381 cells. The presence of collagen on HA surfaces (C-HA) significantly promoted attachment of the organism. HA treated with Type IV collagen bound B. gingivalis cells more effectively than did HA treated with type I collagen. Attachment of two additional strains of B. gingivalis to HA was also promoted by collagen. Binding to type I or type IV C-HA occurred rapidly, and equilibrium was attained within 45 min. B. gingivalis 381 cells also bound to particles of bovine bone collagen, and this appeared to be biphasic. Heating the bacteria abolished their ability to bind to C-HA. Attachment of B. gingivalis 381 cells to HA treated with type I collagen was strongly inhibited by the presence of soluble type I or type IV collagen, or gelatin, but not by the presence of human albumin, salivary proline-rich protein 1, or saliva. Human serum, fibronectin, fibrinogen, certain protease inhibitors, and some peptides were also inhibitory. 3H-fibronectin bound to bovine bone collagen particles and blocked the attachment of 14C-B. gingivalis cells. Mild trypsin treatment of the fibronectin-collagen complex restored its ability to promote 14C-B. gingivalis attachment concomitant with the loss of 3H-fibronectin. We suggest that elevated levels of proteases in the gingival sulcus, such as are associated with poor oral hygiene and gingivitis, might remove fibronectin and expose collagen molecules in the basement membrane, thereby promoting the attachment of B. gingivalis cells and facilitating their invasion into gingival tissues.
Journal of Dental Research, Vol. 67, No. 8,
1075-1080 (1988)
DOI: 10.1177/00220345880670080301
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
R.M. Love and H.F. Jenkinson
INVASION OF DENTINAL TUBULES BY ORAL BACTERIA
Critical Reviews in Oral Biology & Medicine,
March 1, 2002;
13(2):
171 - 183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. DOUNGUDOMDACHA, A. RAWLINSON, and C.W. I. DOUGLAS
Enumeration of Porphyromonas gingivalis, Prevotella intermedia and Actinobacillus actinomycetemcomitans in subgingival plaque samples by a quantitative-competitive PCR method
J. Med. Microbiol.,
October 1, 2000;
49(10):
861 - 874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Love, M. D. McMillan, Y. Park, and H. F. Jenkinson
Coinvasion of Dentinal Tubules by Porphyromonas gingivalis and Streptococcus gordonii Depends upon Binding Specificity of Streptococcal Antigen I/II Adhesin
Infect. Immun.,
March 1, 2000;
68(3):
1359 - 1365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Amano, T. Nakamura, S. Kimura, I. Morisaki, I. Nakagawa, S. Kawabata, and S. Hamada
Molecular Interactions of Porphyromonas gingivalis Fimbriae with Host Proteins: Kinetic Analyses Based on Surface Plasmon Resonance
Infect. Immun.,
May 1, 1999;
67(5):
2399 - 2405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Amano, I. Nakagawa, K. Kataoka, I. Morisaki, and S. Hamada
Distribution of Porphyromonas gingivalis Strains with fimA Genotypes in Periodontitis Patients
J. Clin. Microbiol.,
May 1, 1999;
37(5):
1426 - 1430.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Shibata, M. Hayakawa, H. Takiguchi, T. Shiroza, and Y. Abiko
Determination and Characterization of the Hemagglutinin-associated Short Motifs Found in Porphyromonas gingivalis Multiple Gene Products
J. Biol. Chem.,
February 19, 1999;
274(8):
5012 - 5020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Takeshita, Y. Murakami, Y. Yamashita, M. Ishida, S. Fujisawa, S. Kitano, and S. Hanazawa
Porphyromonas gingivalis Fimbriae Use beta 2 Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 beta Chain Plays a Functional Role in Fimbrial Signaling
Infect. Immun.,
September 1, 1998;
66(9):
4056 - 4060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Amano, S. Shizukuishi, H. Horie, S. Kimura, I. Morisaki, and S. Hamada
Binding of Porphyromonas gingivalis Fimbriae to Proline-Rich Glycoproteins in Parotid Saliva via a Domain Shared by Major Salivary Components
Infect. Immun.,
May 1, 1998;
66(5):
2072 - 2077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.J. Gibbons
Role of Adhesion in Microbial Colonization of Host Tissues: A Contribution of Oral Microbiology
Journal of Dental Research,
March 1, 1996;
75(3):
866 - 870.
[PDF]
|
|
|
|
|
|
|
M. W. Stinson and M. J. Levine
Modulation of Intergeneric Adhesion of Oral Bacteria by Human Saliva
Critical Reviews in Oral Biology & Medicine,
January 1, 1993;
4(3):
309 - 314.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.G. Loos and D.W. Dyer
Restriction Fragment Length Polymorphism Analysis of the Fimbrillin Locus, fimA, of Porphyromonas gingivalis
Journal of Dental Research,
May 1, 1992;
71(5):
1173 - 1181.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Li, R.P. Ellen, C.I. Hoover, and J.R. Felton
Association of Proteases of Porphyromonas (Bacteroides) gingivalis with its Adhesion to Actinomyces viscosus
Journal of Dental Research,
February 1, 1991;
70(2):
82 - 86.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R.J. Gibbons
Bacterial Adhesion to Oral Tissues: A Model for Infectious Diseases
Journal of Dental Research,
May 1, 1989;
68(5):
750 - 760.
[Abstract]
[PDF]
|
|
|
|
|
