This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Lindemann, R.A. Articles by Wolinsky, L.E. Search for Related Content PubMed PubMed Citation Articles by Lindemann, R.A. Articles by Wolinsky, L.E. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB GLUTARALDEHYDE Social Bookmarking                         What's this?

Induction of Activated Lymphocyte Killing by Bacteria Associated with Periodontal Disease

Section of Oral Diagnosis, Oral Medicine, Oral Pathology, , UCLA School of Dentistry, Center for the Health Sciences, Los Angeles, California 90024

K.T. Miyasaki

Section of Periodontics, , UCLA School of Dentistry, Center for the Health Sciences, Los Angeles, California 90024

L.E. Wolinsky

Section of Oral Biology and the Dental Research Institute, UCLA School of Dentistry, Center for the Health Sciences, Los Angeles, California 90024

Complex interactions occur among host defense cells during bacterial infection. Bacteria and bacterial products may enhance or inhibit the effector and regulatory activity of human lymphocytes. Accordingly, we tested the ability of human periodontal pathogens to activate peripheral blood lymphocytes using standard ⁵¹chromium-release assays to measure lymphocyte-mediated cytolysis. Human adherent-cell depleted peripheral blood lymphocytes (PBL) with the addition of glutaraldehyde-fixed bacteria at a 5:1 bacteria:lymphocyte ratio were incubated at 37°C for 24 hr in RPMI 1640 medium. Six of eight bacteria tested significantly augmented lymphocyte killing of the natural killer (NK) cell-sensitive human erythroleukemia cell line K562. E. corrodens, representing activating bacteria, was also able to induce the killing of NK-resistant targets (M14, Raji), comparable with induction by interleukin-2. Lipopolysaccharides extracted from A. actinomycetemcomitans strains, when incubated with PBL, were able to enhance cytotoxicity without the presence of whole bacteria. A majority of cytotoxicity was mediated by NK cells bearing Leu-11 and NKH-1 markers.

Journal of Dental Research, Vol. 67, No. 5, 846-850 (1988)
DOI: 10.1177/00220345880670051001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. Mathur and B.S. Michalowicz Cell-Mediated Immune System Regulation in Periodontal Diseases Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(1): 76 - 89. [Abstract] [Full Text] [PDF]