Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Tamagawa, H.
Right arrow Articles by Tsunemitsu, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tamagawa, H.
Right arrow Articles by Tsunemitsu, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*SODIUM LAURYL SULFATE
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Purification and Some Properties of Fucosyltransferase in Human Parotid Saliva

H. Tamagawa

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

E. Inoshita

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

T. Takeshita

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

M. Takagaki

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

S. Shizukuishi

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

A. Tsunemitsu

Department of Preventive Dentistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

Fucosyltransferase was purified from human parotid saliva by affinity chromatography on GDP-hexanolamine Sepharose, followed by chromatofocusing on PBE 94 exchanger gel. The purified enzyme had the N-acetylglucosaminide {alpha}1->4, the N-acetylglucosaminide {alpha}1->3, and the glucoside {alpha}1->3 fucosyltransferase activities. The molecular weight of the purified enzyme was estimated to be approximately 20,000. These enzyme activities showed identical pH and divalent metal ion dependencies and identical rates of inactivation upon being heated. The paper chromatographic analysis of the fucosylated products by the purified enzyme and the susceptibility of these products to linkage-specific fucosidase digestion indicated that the transferase formed the Fuc {alpha}1->4GlcNAc, Fuc {alpha}1->3GlcNAc, and Fuc {alpha}1->3Glc linkages.

Journal of Dental Research, Vol. 66, No. 1, 72-77 (1987)
DOI: 10.1177/00220345870660011601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. S. Cameron, D. Szczepaniak, and B. W. Weston
Expression of Human Chromosome 19p alpha(1,3)-Fucosyltransferase Genes in Normal Tissues
J. Biol. Chem., August 25, 1995; 270(34): 20112 - 20122.
[Abstract] [Full Text] [PDF]