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Journal of Dental Research
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Separation and Properties of Rabbit Buccal Mucosal Wound Hyaluronidase

C.N. Bertolami

Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Fruit Street, Boston, Massachusetts 02114

R.H. Day

Harvard School of Dental Medicine, Boston, Massachusetts

D.G. Ellis

Shriners Burns Institute

This study establishes the existence of a mammalian buccal mucosal wound hyaluronidase (hyaluronate 4-glycohydrolase; EC 3.2.1.35) having properties distinct from those of the endogenous lysosomal hyaluronidase of normal (uninjured) buccal mucosa. A time-dependent change in hyaluronidase activity was measured, with the highest specific activity occurring on post-wound day 4 (7.7 ± 1.3 unitslmg protein), followed by consecutive decreases until activity was no longer discernible by day 21. Mucosal wound hyaluronidase closely resembled a previously described integumentary wound endoglycosidase in terms of a high pH optimum (5.0-6.0), distinct (but non-exclusive) substrate preference for hyaluronic acid, and ability to generate saturated depolymerization products by an endoglycosidic hydrolysis.

Journal of Dental Research, Vol. 65, No. 6, 939-944 (1986)
DOI: 10.1177/00220345860650061701
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