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A Comparison of Some of the Permeability Characteristics of Intact and Tape-stripped Hamster Cheek Pouches in vitroDepartment of Oral Pathology, School of Clinical Dentistry, University of Sheffield, 31 Claremont Crescent, Sheffield, S10 2TA, United Kingdom
Department of Physiology, University of Sheffield, Biology Building, Western Bank, Sheffield, S10 2TN, United Kingdom
Department of Oral Pathology, School of Clinical Dentistry, University of Sheffield, 31 Claremont Crescent, Sheffield, S10 2TA, United Kingdom Cheek pouches were removed from anesthetized hamsters and were either tape-stripped or left intact and then mounted in Ussing chambers, where they were bathed in Krebs-Ringer bicarbonate saline.
A potential difference could be measured across all pouches and was stable for over an hour across the unstripped pouches. The electrical resistance across the unstripped pouches was high (8960 Reflection coefficients of urea, ethanol, and glycerol were derived by means of streaming potentials. Values for intact pouches were all close to 1 and for tape-stripped pouches were 0.938, 0.822, and 0.509, respectively. Permeability coefficients for urea and ethanol across intact and tape-stripped pouches were, respectively, 12.5 x 10-9 cm/min; 27.6 x 10-9 cm/min; 2325 x 10 -9 cmlmin ; and 28471 x 10-9 cmlmin. The hamster cheek pouch has a high electrical resistance; it is an impermeable structure in which the keratin layer appears to provide a major barrier to the passage of water, ions, and certain solutes.
Journal of Dental Research, Vol. 65, No. 5,
673-676 (1986) This article has been cited by other articles:
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.cm2), whereas the resistance across the tape-stripped pouches was significantly lower (p<0.01), suggesting that these pouches are likely to be more permeable than the unstripped pouches. 
