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Correlated Observations and Analysis of Maturation-ameloblast Morphology and Enamel Mineralization

The First Department of Oral Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142, Japan

R. Takiguchi

The First Department of Oral Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142, Japan

S. Higashi

The Second Department of Oral Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142, Japan

T. Sasaki

Department of Oral Biology and Pathology, School of Dental Medicine, Health Sciences Center, State University of New York at Stony Brook, New York 11794-8700

P.R. Garant

Department of Oral Biology and Pathology, School of Dental Medicine, Health Sciences Center, State University of New York at Stony Brook, New York 11794-8700

A combined HCI-collagenase digestion technique and scanning electron microscopy were used to isolate the enamel organ and to confirm the presence of maturation ameloblasts of both ruffle-ended (RA) and smooth-ended (SA) types on maturing enamel in kitten permanent tooth germs. EDTA perfusion of animals fixed with aldehyde produced two or three belt-like shallow grooves (from 30 to 100 µm wide) running horizontally through the maturing enamel surface, coinciding closely with the SA distribution pattern. In animals that had been perfusion-fixed with unbuffered osmium tetroxide containing 2.5% potassium pyroantimonate, SEM-EDX analysis detected K in a superficial enamel layer overlaid by the SA layer. Potassium concentration decreased gradually toward the deeper layers. Very little K penetrated the enamel under the RA layer. Energy-dispersive x-ray analysis of Ca and P concentrations in the enamel revealed an even distribution of these elements throughout the superficial layer of maturing enamel. These results suggest that the SA layer forms an access route for K and EDTA and that, in spite of the obvious morphological and functional differences between RA and SA, the maturing enamel surfaces overlaid by these two cell types show similar degrees of mineralization.

Journal of Dental Research, Vol. 65, No. 5, 669-672 (1986)
DOI: 10.1177/00220345860650050701


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