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Basic Biological Sciences Isolation and Growth of Human Periodontal Ligament Cells in vitro

Department of Histology, Department of Endodontics, University of Illinois, College of Dentistry, 801 S. Paulina, Chicago, Illinois 60612

G. Carr

Department of Histology, Department of Endodontics, University of Illinois, College of Dentistry, 801 S. Paulina, Chicago, Illinois 60612

J.C. Daniel

Department of Histology

The periodontal ligaments from single human teeth were dissected free of the root surface and plated into culture dishes. In each case two populations of cells were obtained: fibroblasts and epithelial cells. These two populations were separated and grown in culture. The epithelial nature of the epithelioid-appearing cells was confirmed by staining for keratin, exclusively an epithelial cell protein.

Journal of Dental Research, Vol. 64, No. 8, 1026-1030 (1985)
DOI: 10.1177/00220345850640080101


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