Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Yoshie, H.
Right arrow Articles by Hara, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshie, H.
Right arrow Articles by Hara, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Effects of a Fusobacterium nucleatum Extract on Immunoregulation in Mice

H. Yoshie

Department of Periodontology, School of Dentistry

T. Mitsuma

College of Bio-medical Technology, Niigata University, Niigata 951, Japan

K. Kozima

College of Bio-medical Technology, Niigata University, Niigata 951, Japan

K. Hara

Department of Periodontology, School of Dentistry

The purpose of this study was to examine the effect of an extract of Fusobacterium nucleatum on humoral and cell-mediated immunity and phagocyte functions in mice.

The extract was obtained from the heat-inactivated supernatant of sonicated F. nucleatum. Mouse splenocytes were cultured with sheep red blood cells (SRBC), and antibody formation was assayed by counting the plaque-forming cells (PFC). After C3H mouse skin was grafted onto the BALB/c mouse, cytotoxicity of BALB/c mouse splenocytes to L-cells was enumerated, T- and B-cell blastogenesis was determined by the uptake of 3H-thymidine. In vivo phagocyte functions were measured using carbon clearance test, bactericidal, and acid phosphatase activity assays.

The number of SRBC-specific and non-specific PFC in splenocytes stimulated with the extract increased 2.5-8. 7-fold over that of unstimulated splenocytes. Cytotoxicity of splenocytes from the extract-injected mice decreased in a dose-dependent fashion at 12 days after grafting. T cell mitogenicity of the extract was observed. The carbon clearance rate was reduced, and bactericidal and acid phosphatase activities were elevated by the extract injection of the mice.

In conclusion, F. nucleatum extract has an adjuvant effect on humoral immunity, and a suppressive effect on cell-mediated immunity and activated phagocyte functions.

Journal of Dental Research, Vol. 64, No. 3, 431-436 (1985)
DOI: 10.1177/00220345850640030701
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
G.J. Seymour
Invited Review: Possible Mechanisms Involved in the Immunoregulation of Chronic Inflammatory Periodontal Disease
Journal of Dental Research, January 1, 1987; 66(1): 2 - 9.
[Abstract] [PDF]