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Glycylprolyl Dipeptidylaminopeptidase from Bacteroides gingivalis

Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271, Japan

M. Hayakawa

Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271, Japan

S. Murai

Department of Periodontology, Nihon University School of Dentistry, Kanda-surugadai, Tokyo 101, Japan

H. Takiguchi

Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271, Japan

Dipeptidyl aminopeptidase activity was found in the culture medium of Bacteroides gingivalis 381. The enzyme, hydrolyzing glycylprolyl-4-methylcoumaryl-7-amide, was purified 750-fold from culture medium by ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE Bio Gel A column chromatography. The molecular weight, determined by gel filtration, was approximately 160,000. The isoelectric point of the enzyme, estimated by isoelectric focusing using polyacrylamide disk gel electrophoresis, was about pH 6.2. The optimum pH of the enzyme was about 8.0, and the Km value was 0.05 mM. The enzyme activity was strongly inhibited by phenylmethylsulfonylfluoride and diisopropylfluorophosphate. The purified enzyme specifically cleaved glycylprolyl dipeptide from partially digested type I collagen.

Journal of Dental Research, Vol. 64, No. 2, 106-111 (1985)
DOI: 10.1177/00220345850640020201


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