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Micro-analytical Determination of pH, Calcium, and Phosphate in Plaque Fluid
C.A.N. Rankine
Harvard School of Dental Medicine, Boston, Massachusetts 02115 (present address: LSU School of Dentistry, New Orleans, Louisiana 70119)
E.C. Moreno
Forsyth Dental Center, 140 The Fenway, Boston, Massachusetts 02115 (present address: LSU School of Dentistry, New Orleans, Louisiana 70119)
G.L. Vogel
ADAHF Research Unit at the National Bureau of Standards, Gaithersburg, Maryland 20899
H.C. Margolis
Micro-analytical techniques for the determination of calcium, phosphate, and pH in a small volume (<0.25 µl) of plaque fluid are described and evaluated. The accuracy and the precision of the techniques were compared with those for standard macrotechniques applied to a large pooled plaque fluid sample. The results obtained for the micro-analysis of pooled plaque fluid were in excellent agreement with those obtained by macromethods. The described techniques were also used to analyze plaque fluid obtained from single quadrants of the oral cavities of five individuals. In this fashion, it was determined that, although a significant variation in plaque fluid composition exists between the quadrants, a greater variation exists between subjects. Analyses of plaque fluid obtained from six individuals, following sucrose exposure, were also conducted. The pH value of the fluid changed with time, following a typical Stephan curve, with a minimum value occurring between 15 and 30 minutes;following this, the pH increased to a value near that for resting plaque. An inverse relationship between pH and calcium and phosphorus concentrations was observed. It is noted that the described techniques are sensitive enough to carry out the above analyses both accurately and precisely using plaque obtained from a single quadrant.
Journal of Dental Research, Vol. 64, No. 11,
1275-1280 (1985)
DOI: 10.1177/00220345850640110401
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