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Journal of Dental Research
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Factors Involved in Artificial Caries Induction by Oral Streptococci in Extracted Human Teeth

H.W. Kaufman

Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794

J.J. Pollock

Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794

J. Murphy

Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794

S. Lunardi

Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794

J. Vlack

Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794

This study assesses the abilities of S. mutans GS5 and BHT and S. sanguis G9B to produce subsurface lesions on smooth surfaces of extracted human teeth and examines factors which might be responsible for any differences encountered. Teeth were incubated in Brain Heart Infusion broth containing 2% sucrose and a pure culture of the organism to be tested, the media being changed each day for eight days. Surface and media pH's were measured. The mineral content of both the surface enamel and the subsurface lesions was determined by contact microradiography. Significantly deeper and more demineralized lesions were produced by GS5 than by either BHT or G9B. GS5 also produced a lower surface and medium pH and a more dense coating on the teeth. Similar results were obtained with GS5 and G9B when the BHI broth was replaced with FMC synthetic media. It is concluded that the system described is suitable for studying cariogenic potential and will be useful in measuring the anticariogenicity of suspected therapeutic agents.

Journal of Dental Research, Vol. 63, No. 5, 653-657 (1984)
DOI: 10.1177/00220345840630050901


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J. Arends and J. Christoffersen
Invited Review Article: The Nature of Early Caries Lesions in Enamel
Journal of Dental Research, January 1, 1986; 65(1): 2 - 11.
[Abstract] [PDF]