This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Lagerlöf, F. Articles by Dawes, C. Search for Related Content PubMed PubMed Citation Articles by Lagerlöf, F. Articles by Dawes, C. Social Bookmarking                         What's this?

Salivary Clearance of Sugar and its Effects on pH Changes by Streptococcus mitior in an Artificial Mouth

Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada R3E OW3

R. Dawes

Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada R3E OW3

C. Dawes

Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada R3E OW3

We recently developed a computer model of oral sugar clearance (Dawes, 1983), and an artificial mouth has now been constructed which will allow variation in the salivary parameters identified in the model and the study of their effects on bacterial acid production. Using a thin layer of S. mitior, strain 572, over a miniature Sb electrode to measure bacterial pH changes during sugar clearance from the "mouth", we found that three consecutive sets of "Stephan curves" could be obtained with the same bacteria during one day. In each of several experimental series, one salivary parameter was varied, while other parameters were held constant. The maximum pH decrease and the surface area of the registered Stephan curve ( pH.min) were used as measures of acid production.

The results indicate that the volume of "saliva" in the mouth before and after swallowing, the unstimulated salivary flow rate, and the buffer capacity had significant influences on the extent of the pH changes. Other factors, such as the maximum flow rate, the delay between start of stimulation and maximum flow, the volume of saliva in the mouth at time zero, and the taste threshold for sugar, were of lesser importance, confirming some predictions from the computer model.

Journal of Dental Research, Vol. 63, No. 11, 1266-1270 (1984)
DOI: 10.1177/00220345840630110201


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. K. Hope, A. Petrie, and M. Wilson Efficacy of Removal of Sucrose-Supplemented Interproximal Plaque by Electric Toothbrushes in an In Vitro Model Appl. Envir. Microbiol., February 1, 2005; 71(2): 1114 - 1116. [Abstract] [Full Text] [PDF]

				J. Pratten and M. Wilson Antimicrobial Susceptibility and Composition of Microcosm Dental Plaques Supplemented with Sucrose Antimicrob. Agents Chemother., July 1, 1999; 43(7): 1595 - 1599. [Abstract] [Full Text]

				G.H. Bowden The Role of Microbiology in Models of Dental Caries: Reaction Paper Advances in Dental Research, November 1, 1995; 9(3): 255 - 269. [Abstract] [PDF]

				L.M.D. Macpherson and C. Dawes Effects of Salivary Film Velocity on pH Changes in an Artificial Plaque Containing Streptococcus oralis, After Exposure to Sucrose Journal of Dental Research, September 1, 1991; 70(9): 1230 - 1234. [Abstract] [PDF]

				L.M.D. Macpherson, W.-Y. Chen, and C. Dawes Effects of Salivary Bicarbonate Content and Film Velocity on pH Changes in an Artificial Plaque Containing Streptococcus oralis, After Exposure to Sucrose Journal of Dental Research, September 1, 1991; 70(9): 1235 - 1238. [Abstract] [PDF]

				L.M.C. Collins and C. Dawes The Surface Area of the Adult Human Mouth and Thickness of the Salivary Film Covering the Teeth and Oral Mucosa Journal of Dental Research, August 1, 1987; 66(8): 1300 - 1302. [Abstract] [PDF]

				C. Dawes and S. Watanabe The Effect of Taste Adaptation on Salivary Flow Rate and Salivary Sugar Clearance Journal of Dental Research, March 1, 1987; 66(3): 740 - 744. [Abstract] [PDF]

				F. Lagerlof, A. Oliveby, and J. Ekstrand Physiological Factors Influencing Salivary Clearance of Sugar and Fluoride Journal of Dental Research, February 1, 1987; 66(2): 430 - 435. [Abstract] [PDF]

				F. Lagerlof and C. Dawes Clinical Science: The Effect of Swallowing Frequency on Oral Sugar Clearance and pH Changes by Streptococcus mitior in vivo After Sucrose Ingestion Journal of Dental Research, October 1, 1985; 64(10): 1229 - 1232. [Abstract] [PDF]