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Journal of Dental Research
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Identification of a Cathepsin B-like Protease in the Crevicular Fluid of Gingivitis Patients

D.A. Eisenhauer

Departments of Biochemistry and Periodontics, Medical University of South Carolina, Charleston, South Carolina 29425

R. Hutchinson

Departments of Biochemistry and Periodontics, Medical University of South Carolina, Charleston, South Carolina 29425

T. Javed

Departments of Biochemistry and Periodontics, Medical University of South Carolina, Charleston, South Carolina 29425

J.K. McDonald

Departments of Biochemistry and Periodontics, Medical University of South Carolina, Charleston, South Carolina 29425

Crevicular fluid from gingivitis patients contains significant levels of a cysteine protease which was characterized as the lysosomal protease cathepsin B, as judged by substrate specificity, thiol dependence, pH optimum, kinetic parameters, pH stability, and inhibitor sensitivities. A highly-sensitive fluorometric assay procedure was used to establish the mean level of cathepsin B activity for 25 gingivitis patients.

Journal of Dental Research, Vol. 62, No. 8, 917-921 (1983)
DOI: 10.1177/00220345830620081401
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