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Journal of Dental Research
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Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands

E. Zdebska

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

Y.H. Liau

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

A. Slomiany

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

B.L. Slomiany

Gastroenterology Research Laboratory, Department of Medicine, New York Medical College, Research Center, Metropolitan Hospital, New York, New York 10029

The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3'-phosphoadenosine-5'-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F1-.

Journal of Dental Research, Vol. 62, No. 10, 1026-1030 (1983)
DOI: 10.1177/00220345830620100201


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