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The Isolation of a Family of Cysteine-containing Phosphoproteins from Human Submandibular-Sublingual Saliva

Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, Buffalo, New York 14226

L.A. Tabak

Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, Buffalo, New York 14226

M.J. Levine

Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, Buffalo, New York 14226

I.D. Mandel

School of Dental and Oral Surgery, Columbia University, New York, New York 10032

S.A. Ellison

School of Dental and Oral Surgery, Columbia University, New York, New York 10032

A family of acidic proteins has been isolated from human submandibular-sublingual saliva using Sephadex G-200 gel filtration, DE-52 cellulose chromatography, and immuno-affinity chromatography. These molecules were homogenous in size and by immunological criteria, but exhibited charge heterogeneity. The heterogeneity displayed was not due to gustatory stimulation or degradation during isolation procedures.

Journal of Dental Research, Vol. 61, No. 8, 973-977 (1982)
DOI: 10.1177/00220345820610081101


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