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Journal of Dental Research
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Ultrastructural Alteration of Trypsin- and Pancreatin-separated Embryonic Rabbit Palate Epithelium and Mesenchyme

Richard J. Lieb

Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298

Dominick P. DePaola

Oral Health Research Center, Fairleigh Dickinson University, School of Dentistry, Hackensack, New Jersey 07601

The effects of 30- and 45-minute trypsin- and pancreatin-separation on embryonic rabbit palate epithelium and mesenchyme were studied with the electron microscope. Changes in epithelium included fragmentation of the basal lamina and formation of cytoplasmic blebs associated with the basal cell layer. Changes in mesenchymal cells were first evident at 45 min of incubation and included cell fragmentation, nuclear pyknosis, and dilation of extracellular space. Results indicate a differential susceptibility of mesenchyme to the separating agent.

Journal of Dental Research, Vol. 60, No. 2, 164-170 (1981)
DOI: 10.1177/00220345810600021301
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